Acid Phosphatase 8

Acid Phosphatase  8

Product Details:

  • Shelf Life 18 Months
  • Taste Other
  • Appearance Biochemistry reagent
  • Shape other
  • Usage Biochemistry reagent
  • Physical Form Liquid
  • Other Names Biochemistry reagent
  • Supply Ability : 10000 Kit Per Day
1056 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 1056 INR/Kit

Product Specifications

  • Biochemistry reagent
  • ACC556895
  • Biochemistry reagent
  • Liquid
  • Biochemistry reagent
  • Other
  • 18 Months
  • 100
  • Other
  • Biochemistry reagent
  • Reagents
  • Other
  • other

Trade Information

  • EXW ABPL
  • Paypal, Letter of Credit (L/C), Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empty
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION
  • Auto Zyme Acid Phosphatase is a reagent set for determination of Acid Phosphatase activity based on kinetic method using  a-naphthyl phosphate.
  • Auto Zyme Acid Phosphatase is a single reagent system using one step procedure.
  • Auto Zyme Acid phosphatase can be determined in just 8 minutes at 37 degree C.
  • Auto Zyme Acid Phosphatase is linear up to 75 IU/1.
  • Auto Zyme Acid Phosphatase can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
PRINCIPLE

In acidic pH of buffer system, Acid Phosphatase hydrolyses a-naphthyl phosphate to a-naphthol and phosphate. The a-naphthol is then coupled with Diazotized Fast Red TR to form a Diazo dye which has strong absorbance at 405 nm. The increase in absorbance is directly proportional to the level of acid phosphatase in serum.

The addition of L-Tartrate inhibits prostatic acid phosphatase but does not inhibit other isoenzyme.

The activity of Prostatic Acid Phosphatase is obtained by subtracting the result of Non prostatic Acid phosphatase (Determination with Tartrate) from Total Acid Phosphatase (without tartrate).

PREPARATION OF  WORKING SOLUTION

  • Total Acid Phosphatase Working Solution
  • Dissolve the contents of the substrate bottle with Diluent for Total ACP as per instructions indicated on individual bottle label to prepare working solution.
  • Non Prostatic Phosphatase Working Solution
  • Dissolve the contents of the substrate bottle with Diluent for Non Prostatic ACP as per instructions indicated on individual bottle label to prepare working solution.
  • The Stabilizer reagent (Acetate Buffer) is ready-to-use as provided.

NOTE : ACP -Acid Phosphatase

REAGENT STORAGE & STABILITY

  • The kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label.
  • The working solutions of  Non Prostatic Acid Phosphatase & Total Acid Phosphatase are stable for 7 days at 2 - 8 degree C.

COMPONENTS & CONCENTRATION OF WORKING SOLUTION

Component

Concentration

 

Total ACP Non Prostatic ACP

Buffer, pH 5.3

80 mmo1/1

80 mmo1/1

a - naphthyl phosphate

 5.0 mmo1/1

 5.0 mmo1/1

Fast Red TR

1.0 mmo1/1

1.0 mmo1/1

Tartrate

150 mmo1/1

 SPECIMEN COLLECTION & PRESERVATION

Serum sample must be completely free from hemolysis. Acid Phosphatase is very unstable at the pH of serum. Immediately after separation of the serum from the clot, stabilize the serum by the addition of 20 ill of stabilizer reagent (Acetate buffer) to every 1 ml of serum. The enzyme activity will be stable for 3 days at 2- 8 Degree C. Oxalate & fluoride as anticoagulants will interfere with the assay

PROCEDURE

  • Reaction type: Kinetic
  • Reaction direction : Increasing
  • Wavelength: 405 nm.
  • Flowcell temperature :37 degree C
  • Zero setting with :Distilled water
  • Delay time: 300 seconds
  • No. of readings: 4
  • Interval: 60 seconds
  • Blank absorbance limit:  0.500
  • Sample volume: 0.1 ml (100 ill)
  • Reagent volume:  1.0 ml
  • Factor: 743
  • Linearity: 75 IU/1
Manual assay procedure

Prewarm at 37 degree C the required amount of working solutions before use.

Perform the assay as given below :

Total Acid Phosphatase

  • Specimen: 0.1 ml (100 (II)
  • Total ACP working solution : 1.0 ml

Non Prostatic Acid Phosphatase

  • Specimen: 0.1 ml (100 gl)
  • Non Prostatic ACP working solution :1.0 ml

For each assay, mix thoroughly and transfer the assay mixture immediately to the thermo stated cuvette and start the stop watch simultaneously. Record the first reading at 300th second and subsequently three more readings with 60 seconds interval at 405 nm.

EXPECTED VALUES

  • Total Acid Phosphatase : up to 4.7 IU/1
  • Prostatic Acid Phosphatase: up to 1.6 IU/1

NOTE :

Since the expected values are affected by age, sex, diet and geographical location, each laboratory is strongly urged to establish its own reference range for this procedure.

PROCEDURE LIMITATIONS

Working reagent is considered unsatisfactory and should not be used if its absorbance exceeds 0.500 at 405 nm. against distilled water.

If the Acid Phosphatase activity is above 75 IU/1 then dilute the specimen suitably with normal saline. In such case the results obtained should be multiplied by dilution factor to obtain correct Acid Phosphatase activity.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

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