ADA 60

ADA 60

Product Details:

  • Shape Cubes
  • Taste Other
  • Other Names Biochemistry Reagent
  • Shelf Life 18 Months
  • Usage Infinite ADA is a ready-to-use, two liquid reagent system. With Infinite ADA, the assay is linear upto 200 U/L.
  • Appearance Biochemistry Reagent
  • Smell Other
  • Supply Ability : 10000 , , Kit Per Day
20423 INR/Kit
X

Price And Quantity

  • 20423 INR/Kit
  • 1 , , Kit

Product Specifications

  • Cubes
  • Other
  • Biochemistry Reagent
  • 18 Months
  • ACC441415
  • 100
  • Infinite ADA is a ready-to-use, two liquid reagent system. With Infinite ADA, the assay is linear upto 200 U/L.
  • Biochemistry Reagent
  • Other
  • Other
  • Reagents
  • Biochemistry Reagent
  • Liquid

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 , , Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION 
  •  Infinite ADA is a reagent set for direct quantitative  determination of Adenosine deaminase (ADA) activity in  human serum, plasma and other body fluids. 
  •  Infinite ADA is a ready-to-use, two liquid reagent system. 
  • With Infinite ADA, the assay is linear upto 200 U/L. 
  • Infinite ADA assay can be performed in 11 minutes at  37 C. 
PRINCIPLE

ADA catalyses deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleosidephosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase  (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine  (4 AAP) in the presence of peroxidase (POD) to generate  quinone dye.

denosine + H2O Inosine + NH3 PNP Inosine + Pi Hypoxanthine + Ribose-1-phosphate XOD Hypoxanthine + 2H2O + 2O2 Uric acid + 2 H2O2 POD 2 H2O2 + 4AAP + EHSPT 4 H2O + Quinone dye  

The intensity of the colour developed is directly proportional to the activity of ADA in the specimen and is measured kinetically.  

REAGENT STORAGE,  STABILITY & HANDLING 

  • The reagents R1 & R2 are ready-to-use. 
  • The reagent kit should be stored at 2 Degree- 8 Degree C and is stable till 
  • the expiry date indicated on the label. 
  • Reagent R1 is light-sensitive & to be stored in dark. 
  • Contamination of the reagents should be strictly avoided. 
COMPONENTS & CONCENTRATION OF WORKING SOLUTION 

Component Concentration 

R1 

  • Tris 45mM 
  • PNP 0.08 U/ml 
  • Xanthine oxidase 0.15 U/ml 
  • Peroxidase 0.40 U/ml 
  • Stabilizers, excipients & surface active agents 
 R2 
  • Tris 40mM 
  • Adenosine 8 M 
  • EHSPT 1M
SPECIMEN COLLECTION  & PRESERVATION 

Blood should be collected in a clean dry container. Serum or  heparinized plasma may be used. Do not use citrate or oxalate as anticoagulant. Ideally, venous blood should be  collected & handled anaerobically.

It is recommended to use fresh sample. Serum or plasma,  after prompt separation from cells or clot, should be kept  tightly stoppered. Do not use hemolyzed, contaminated or  turbid samples.  Ideal collection procedure, i.e. disinfecting the site &  collecting with aseptic precautions, should be followed when  other body fluids (Pleural fluid, pericardial fluid, ascitic fluid, cerebrospinal fluid) are tested. 

ADA activity in serum/plasma is stable for 3 days when stored  at 2 Degree - 8 Degree C & in other body fluids for 2 days when stored at 2 Degree - 8 Degree C .  

PROCEDURE

  • Reaction type :  Kinetic 
  • Reaction direction:  .Increasing 
  • Wavelength :  546 nm 
  • Flowcell temperature:  .37 Degree C 
  • Zero setting with:  Distilled water 
  • Delay time:  300 seconds 
  • No. of readings:   4 
  • Interval :   60 seconds 
  • Sample volume:  10 Ul 
  • R 1 volume:   360 Ul 
  • R 2 volume:  180 Ul 
  • Factor:   1743 
  • Linearity:   200 U/L 

EXPECTED VALUES

The indicative reference values for Indian population are as follows:

 

Normal

Suspect

Strong Suspect

Serum/plasma

 22U/L

22 - 40U/L

 40U/L

Body fluids (Pleural/Pericardial/Ascitic)

 30U/L

30 - 40U/L

40U/L

CSF

 9U/L

9 - 12 U/L

12U/L

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