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ADA Control Low High
12156 INR/Kit
Product Details:
- Other Names Biochemistry Reagent
- Shelf Life 18 Months
- Smell Other
- Usage Infinite ADA is a ready-to-use, two liquid reagent system. With Infinite ADA, the assay is linear upto 200 U/L.
- Appearance Biochemistry Reagent
- Shape other
- Taste Other
- Supply Ability : 10000 Kit Per Day
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Price And Quantity
- 12156 INR/Kit
- 1 Kit
Product Specifications
- Biochemistry Reagent
- Liquid
- 100
- Other
- other
- Reagents
- Other
- ACC90599
- Other
- Infinite ADA is a ready-to-use, two liquid reagent system. With Infinite ADA, the assay is linear upto 200 U/L.
- Biochemistry Reagent
- Biochemistry Reagent
- 18 Months
Trade Information
- EXW ABPL
- Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
- 10000 Kit Per Day
- 2 Days
- Yes
- packaging : Empity
- Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
- All India
- certification : iso
Product Description
INTRODUCTION
- Infinite ADA is a reagent set for direct quantitative determination of Adenosine deaminase (ADA) activity in human serum, plasma and other body fluids.
- Infinite ADA is a ready-to-use, two liquid reagent system.
- With Infinite ADA, the assay is linear upto 200 U/L.
- Infinite ADA assay can be performed in 11 minutes at 37 C.
PRINCIPLE
ADA catalyses deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleosidephosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4 AAP) in the presence of peroxidase (POD) to generate quinone dye.
denosine + H2O Inosine + NH3 PNP Inosine + Pi Hypoxanthine + Ribose-1-phosphate XOD Hypoxanthine + 2H2O + 2O2 Uric acid + 2 H2O2 POD 2 H2O2 + 4AAP + EHSPT 4 H2O + Quinone dye
The intensity of the colour developed is directly proportional to the activity of ADA in the specimen and is measured kinetically.
REAGENT STORAGE, STABILITY & HANDLING
- The reagents R1 & R2 are ready-to-use.
- The reagent kit should be stored at 2 Degree- 8 Degree C and is stable till
- the expiry date indicated on the label.
- Reagent R1 is light-sensitive & to be stored in dark.
- Contamination of the reagents should be strictly avoided.
COMPONENTS & CONCENTRATION OF WORKING SOLUTION
Component Concentration
R1
- Tris 45mM
- PNP 0.08 U/ml
- Xanthine oxidase 0.15 U/ml
- Peroxidase 0.40 U/ml
- Stabilizers, excipients & surface active agents
R2
- Tris 40mM
- Adenosine 8 M
- EHSPT 1M
SPECIMEN COLLECTION & PRESERVATION
Blood should be collected in a clean dry container. Serum or heparinized plasma may be used. Do not use citrate or oxalate as anticoagulant. Ideally, venous blood should be collected & handled anaerobically.
It is recommended to use fresh sample. Serum or plasma, after prompt separation from cells or clot, should be kept tightly stoppered. Do not use hemolyzed, contaminated or turbid samples. Ideal collection procedure, i.e. disinfecting the site & collecting with aseptic precautions, should be followed when other body fluids (Pleural fluid, pericardial fluid, ascitic fluid,
cerebrospinal fluid) are tested.
ADA activity in serum/plasma is stable for 3 days when stored at 2 Degree - 8 Degree C & in other body fluids for 2 days when stored at 2 Degree - 8 Degree C .
PROCEDURE
- Reaction type : Kinetic
- Reaction direction: .Increasing
- Wavelength : 546 nm
- Flowcell temperature: .37 Degree C
- Zero setting with: Distilled water
- Delay time: 300 seconds
- No. of readings: 4
- Interval : 60 seconds
- Sample volume: 10 Ul
- R 1 volume: 360 Ul
- R 2 volume: 180 Ul
- Factor: 1743
- Linearity: 200 U/L
EXPECTED VALUES
The indicative reference values for Indian population are as follows:
| | Normal | Suspect | Strong Suspect |
| Serum/plasma | 22U/L | 22 - 40U/L | 40U/L |
| Body fluids (Pleural/Pericardial/Ascitic) | 30U/L | 30 - 40U/L | 40U/L |
| CSF | 9U/L | 9 - 12 U/L | 12U/L |
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Contact Details
ACCUREX BIOMEDICAL PVT. LTD.
- 212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai - 400016, Maharashtra, India
- Phone : 08045479256
- Fax : 91-22-66662223
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