Urea 200

Auto Zyme Urea is a reagent set for determination of Urea / Blood Urea Nitrogen based on enzymatic method using Urease. Auto Zyme Urea is a two reagent system using two step procedure. Auto Zyme Urea is linear up to 350 mg%. The stability of enzyme solution is 6 months at 2 -8 degree C Auto Zyme urea can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.

Total Protein 100

Auto Zyme Total protein is a reagent set for determination of total protein based on Biuret method. Auto Zyme Total protein is a single reagent system, ready-to-use. Auto Zyme Total protein is linear up to 18 gm%. Total protein can be determined in just 5 minutes. Auto Zyme Total protein does not require serum blank for lipemic samples. Auto Zyme Total protein can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. The shelf-life of Auto Zyme Total protein is 18 months.

Acid Phosphatase 8

INTRODUCTION Auto Zyme Acid Phosphatase is a reagent set for determination of Acid Phosphatase activity based on kinetic method using a-naphthyl phosphate. Auto Zyme Acid Phosphatase is a single reagent system using one step procedure. Auto Zyme Acid phosphatase can be determined in just 8 minutes at 37 degree C. Auto Zyme Acid Phosphatase is linear up to 75 IU/1. Auto Zyme Acid Phosphatase can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. PREPARATION OF WORKING SOLUTION Total Acid Phosphatase Working Solution Dissolve the contents of the substrate bottle with Diluent for Total ACP as per instructions indicated on individual bottle label to prepare working solution. Non Prostatic Phosphatase Working Solution Dissolve the contents of the substrate bottle with Diluent for Non Prostatic ACP as per instructions indicated on individual bottle label to prepare working solution. The Stabilizer reagent (Acetate Buffer) is ready-to-use as provided. NOTE : ACP -Acid Phosphatase

Chloride 100

INTRODUCTION Auto Zyme Chloride is a reagent set for determination of chloride in serum/plasma based on Mercuric thiocyanate method. Auto Zyme Chloride is a single reagent system, ready-to-use. Auto Zyme Chloride estimates chloride in just 1 minute. Auto Zyme Chloride is linear up to 130 mmol/l. Auto Zyme Chloride can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. The shelf-life of Auto Zyme Chloride is 15 months REAGENT STORAGE & STABILITY The reagents are for in vitro diagnostic use. Thiocyanate reagent and standard should be stored at temperature indicated on the bottle label. COMPONENTS & CONCENTRATION OF WORKING SOLUTION Component Concentration Mercuric thiocyanate 2.0 mmol/l Mercuric chloride 0.8 mmol/l Ferric nitrate 20.0 mmol/l Nitric acid 45.0 mmol/l Stabilizers, surface active agent and inactive ingredients

Gamma GT 10

INTRODUCTION Auto Zyme Gamma-GT is a reagent set for determination of gamma glutamyl transferase (GGT) in serum and plasma based on IFCC method. Auto Zyme Gamma-GT is a ready-to-use, two liquid reagent system, using single step reconstitution. Auto Zyme Gamma-GT estimates GGT activity in just 3 minutes. Auto Zyme Gamma-GT is linear up to 1000 IU/1. Auto Zyme Gamma-GT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. Programmed can be designed for any specific analyzer upon request. Auto Zyme Gamma-GT is stable till expiry at 2- 8 degree C. PREPARATION OF WORKING SOLUTION R1 : Buffer reagent is ready-to-use. R2 : Transfer contents of diluent bottle R2(b) into substrate bottle R2(a) as per the instructions indicated on the label. Mix gently by inversion. Prepare working solution by mixing Reagent R1 and Reagent R2 in the ratio 4 : 1 as per requirement. Note : Over a period of time, the reagent R2 after reconstitution may develop a yellow colour. This is expected and does not affect the performance of the assay.

G6PDH Quantitative Kinetic 12

INTRODUCTION Auto Zyme NEW G6PDH is a reagent set for quantitative determination of G6PDH activity in erythrocytes by kinetic method. Auto Zyme NEW G6PDH can be used on any semi-automated analyzer. PRINCIPLE G6PDH present in the erythrocytes (RBCs) is extracted by lysing the cells using lysing reagent. The extracted enzyme catalyzes the following reaction : G6PDH Glucose-6-Phosphate + NADP P 6-Phosphogluconate + NADPH The conversion of co-enzyme NADP to NADPH is proportional to the concentration of G6PDH activity in the sample and is measured at 340 nm as rate of increase in absorbance.

G6PDH Qualitative Dye 12

INTRODUCTION Auto Zyme NEW G6PDH (Qualitative-Dye) is a reagent set for qualitative determination (screening) of G6PDH deficiency in erythrocytes by decolourisation method. Auto Zyme NEW G6PDH (Qualitative-Dye) is a reagent set with simple procedure and easy technique. Auto Zyme NEW G6PDH (Qualitative-Dye) is available in convenient pack-size of 12 tests. PRINCIPLE Glucose-6-Phosphate Dehydrogenase (G6PDH) present in haemolysate acts on substrates, Glucose-6-Phosphate and NADP, giving NADPH, which in presence of PMS (Phenazine methosulfate) decolorises blue coloured indophenol dye (DCPIP) leaving behind colour only due to haemolysate. The rate of reaction is proportional to G6PDH present, hence time required for decolorisation is inversely proportional to G6PDH activity in the haemolysate.

GOT 30

INTRODUCTION Auto Zyme GOT is a reagent set for determination of GOT (AST) activity in serum or plasma. The reaction is based on UV-Kinetic method recommended by the International Federation of Clinical Chemistry (IFCC). Auto Zyme GOT is a single reagent system, using one step procedure. Auto Zyme GOT is a High Stability Reagent. Auto Zyme GOT is linear up to 400 IU/1. Auto Zyme GOT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. GOT activity can be determined in 21/2 minutes. PREPARATION OF WORKING SOLUTION Reconstitute reagents as per instruction on individual bottle label to prepare working solution. Mix by gentle swirling or inversion. Do not shake vigorously. REAGENT STORAGE & STABILITY The reagent kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label. The GOT working solution is stable for 60 days at 2-8 degree C. Do not freeze the working solution.

GPT 60

INTRODUCTION Auto Zyme GPT is a reagent set for determination of GPT (ALT) activity in serum or plasma. The reaction is based on UV-Kinetic method recommended by the International Federation of Clinical Chemistry (IFCC). Auto Zyme GPT is a single reagent system, using one step procedure. Auto Zyme GPT is a High Stability Reagent. Auto Zyme GPT is linear up to 400 IU/1. Auto Zyme GPT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. GPT activity can be determined in TA minutes. The shelf-life of Auto Zyme GPT is 18 months. Although serum is preferred, plasma can also be used. Anticoagulants such as heparin and EDTA can be used. Blood samples may be collected any time, although morning samples are preferred. Samples with any visible hemolysis are not acceptable. Samples are stable for a week at 2 - 8 degree C and for one month at -10 degree C. Samples should be brought to room temperature prior to use.

Phosphorus 100

INTRODUCTION Auto Zyme Phosphorus is a reagent set for determination of Inorganic phosphorus in serum based on UV - End Point method using Ammonium molybdate. Auto Zyme Phosphorus is a single reagent system, ready-to-use. Auto Zyme Phosphorus is linear up to 20 mg%. Auto Zyme Phosphorus estimates phosphorus in just five minutes. Auto Zyme Phosphorus can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. The shelf-life of Auto Zyme Phosphorus is 18 months.

Alkaline Phosphatase 60

GOT 200

INTRODUCTION Auto Zyme GOT is a reagent set for determination of GOT (AST) activity in serum or plasma. The reaction is based on UV-Kinetic method recommended by the International Federation of Clinical Chemistry (IFCC). Auto Zyme GOT is a single reagent system, using one step procedure. Auto Zyme GOT is a High Stability Reagent. Auto Zyme GOT is linear up to 400 IU/1. Auto Zyme GOT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. GOT activity can be determined in 21/2 minutes. PRINCIPLE The conversion of NADH to WAD+ is proportional to the concentration of GOT in serum and is measured at 340 nm. as rate of decrease in absorbance. Abbreviations GOT : Glutamate Oxaloacetate Transaminase AST: Aspartate Transaminase

GPT 200

INTRODUCTION Auto Zyme GPT is a reagent set for determination of GPT (ALT) activity in serum or plasma. The reaction is based on UV-Kinetic method recommended by the International Federation of Clinical Chemistry (IFCC). Auto Zyme GPT is a single reagent system, using one step procedure. Auto Zyme GPT is a High Stability Reagent. Auto Zyme GPT is linear up to 400 IU/1. Auto Zyme GPT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. GPT activity can be determined in TA minutes. The shelf-life of Auto Zyme GPT is 18 months. Although serum is preferred, plasma can also be used. Anticoagulants such as heparin and EDTA can be used. Blood samples may be collected any time, although morning samples are preferred. Samples with any visible hemolysis are not acceptable. Samples are stable for a week at 2 - 8 degree C and for one month at -10 degree C. Samples should be brought to room temperature prior to use.

Albumin 100

Introduction Auto Zyme Albumin is a reagent set for determination of Albumin in serum or plasma based on BCG method. Auto Zyme Albumin is a single reagent system, ready-to-use. Auto Zyme Albumin is a linear upto 6 gm%. Albumin can be determined in just 1 minute at room temperature. Auto Zyme Albumin can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request. The shelf-life of Auto Zyme Albumin is 18 months. SPECIMEN COLLECTION & PRESERVATION Blood should be collected in a clean dry container. Plastic or siliconized container should be avoided as it may prolong clotting time. Serum is preferred but plasma can also be used. For plasma separation anticoagulants like EDTA or Heparin can be used. Avoid venous stasis. In absence of bacterial contamination, aloumin is stable in sample for 1 week at room temperature and 1 month at 2-8 degree

Albumin 300

Introduction Auto Zyme Albumin is a reagent set for determination of Albumin in serum or plasma based on BCG method. Auto Zyme Albumin is a single reagent system, ready-to-use. Auto Zyme Albumin is a linear upto 6 gm%. Albumin can be determined in just 1 minute at room temperature. Auto Zyme Albumin can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request. The shelf-life of Auto Zyme Albumin is 18 months. PRINCIPLE Serum albumin in the presence of Bromocresol-green under acidic condition forms a green coloured complex. The absorbance of this complex is proportional to the albumin concentration in serum or plasma. PREPARATION OF WORKING SOLUTION The reagent is ready-to-use. REAGENT STORAGE & STABILITY The reagents are for in vitro diagnostic use. BCG reagent and standard should be stored at temperature indicated on the bottle label.

Bilirunin (T And D) 200

brand name : ACCUREX BIOMEDICAL

BUN 200

brand name : ACCUREX BIOMEDICAL

Calcium 100

INTRODUCTION Auto Zyme Calcium is a reagent set for determination of calcium based on the colorimetric method using o-Cresol phthalein Complex one. Auto Zyme Calcium is a single reagent system, using one step procedure. Auto Zyme Calcium is linear u pto 15 mg% Auto Zyme Calcium is a High Stability Reagent. Auto Zyme Calcium can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. Auto Zyme Calcium has one step reconstitution. It involves mixing of CPC and Diluent reagent. Calcium can be determined in just 5 minutes. Auto Zyme Calcium has minimum reagent wastage since working solution can be prepared according to the need. Interference of Mg and Fe is negligible. Lipemic samples do not require any special treatment.

Calcium Arsenazo III 150

INTRODUCTION Auto Zyme Calcium Arsenazo III is a reagent set for determination of total calcium concentration in serum based on the colorimetric method using Arsenazo Ill. Auto Zyme Calcium Arsenazo III is a ready-to-use reagent. Auto Zyme Calcium Arsenazo III estimates calcium in just 2 minutes at R.T. (25-30 degree C). Auto Zyme Calcium Arsenazo III is linear up to 15 mg%. Auto Zyme Calcium Arsenazo III can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. The influence of lipids, hemolysis and bilirubin is negligible. REAGENT STORAGE, STABILITY AND HANDLING The kit should be stored at R.T. (25-30 degree C) and is stable till the expiry date indicated on the label. Contamination of the reagent should be strictly avoided. Protect the reagent from direct light. Should the reagent develop turbidity discard the reagent

Creatinine 200

Introduction AutoPure Creatinine is a reagent kit for direct quantitative determination of Creatinine in human serum and urine based on enzymatic method. AutoPure Creatinine is ready to use , two liquid reagent system. With AutoPure Creatinine, the assay is linear upto 200 mg/dl. Clinical Significance Creatinine is a chemical waste molecule that is generated from muscle metabolism. Creatinine is produced from Creatinine, a molecule of major importance for energy production in muscles. Approximately 2 % of the bodys Creatinine is converted to Creatinine every day. Creatinine is transported through the bloodstream to the kidneys. The kidneys filter out most of the Creatinine and dispose . It of in the urine. The kidneys maintain the blood Creatinine in a normal range. Creatinine has been found to be a fairly reliable indicator of kidney function. As the kidney become impaired the Creatinine level in the blood will rise. Abnormally high levels of Creatinine thus warn of posssible malfunction or failure of the kidneys.

Glucose STAT 1000

INTRODUCTION Autozyme Glucose is a reagent set for determination of True Glucose, based on enzymatic method; using Glucose, oxidase and Peroxidase. Autozyme Glucose is a ready-to-use reagent. AutozymeGlucose estimates glucose in 7 minutes at 37 Degree C or15 minutes at R.T. by end point method. Autozyme Glucose is linear up to 500 mg%. Autozyme Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request. Autozyme (as an anticoagulant up to 10 mg/ml blood) does not effect glucose assay. The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Haemoglobin is negligible. PRINCIPLE Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of Peroxidase (POD), oxidatively couples with 4-aminoantipyrine and phenol to produce red quinoneimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.). The intensity of the colour complex is directly proportional to the concentration of glucose in specimen.

Diluent Glucose STAT 1000

INTRODUCTION Auto Zyme STAT Glucose is a reagent set for determination of True Glucose based on enzymatic method using Glucose oxidase and Peroxidase. Auto Zyme STAT Glucose estimates glucose in just one minute using an initial rate method and in 7 minutes by end-point method. Auto Zyme STAT Glucose pack sizes 10 x 100 ml and 4 x 500 ml are provided with 2 standards of 100 mg% and 300 mg%. Auto Zyme STAT Glucose is Linear up to 700 mg% glucose for kinetic assay procedure and 500 mg% using end-point procedure. Auto Zyme STAT Glucose is a High Stability Reagent. Auto Zyme STAT Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. Auto Zyme STAT Glucose has one step reconstitution. It involves the mixing of ENZYME and DILUENT. Sodium Fluoride (as an anticoagulant up to 10 mg/ml blood) does not effect glucose assay. The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible. Very small volume of serum or plasma is required for assay. The shelf-life of Auto Zyme STAT Glucose is 18 months. PRINCIPLE Glucose oxidase (GOD) converts Glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of Peroxidase (POD), oxidatively couples with 4-aminoantipyrine / phenol to produce red quinonimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.). The intensity of the colour complex is directly proportional to the Glucose in specimen.

Diluent Glucose STAT 2000

PREPARATION OF WORKING SOLUTION 4 x 500 ml (Code GU-3) Transfer the content of one vial of Enzyme into one bottle of Diluent and mix by gentle swirling or inversion. Do not shake vigorously. 10 X 100 ml (Code GU-2) Transfer 100 mL. Diluent into working solution bottle. Now add contents of one vial of Enzyme into it. Mix by gentle swirling or inversion. Do not shake vigorously. REAGENT STORAGE & STABILITY Stability of reconstituted working reagent is printed on the bottle label. (DO NOT FREEZE). The working solution should be stored in the dark bottle (diluent bottle or the working solution bottle) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light. COMPONENTS & CONCENTRATION OF WORKING SOLUTION Component Concentration Phosphate Buffer pH 7.0 170 mmo1/1 Glucose oxidase 15000 IU/1 Peroxidase 1500 IU/1 4-aminoantipyrine 0.28 mmo1/1 Phenol 16 mmo1/1 SPECIMEN COLLECTION & PRESERVATION Blood should be collected in a clean dry container. Serum or plasma should be separated from the cells at the earliest possible (within 30 minutes), as the rate of glycolysis is approximately 7 mg% per hour at room temperature. For plasma separation following anticoagulants may be used. EDTA 2 mg/ml of blood CITRATE 6 mg/ml of blood HEPARIN 200 Wml of blood OXALATE 3 mg/ml of blood SODIUM FLUORIDE 10 mg/ml of blood

Glucose STAT 2000

INTRODUCTION infinite Liquid STAT Glucose is a reagent set for determination of True Glucose, based on enzymatic method; using Glucose, oxidase and Peroxidase. infinite Liquid STAT Glucose is a ready-to-use reagent. Infinite Liquid STAT Glucose estimates glucose in 7 minutes at 37 Degree C or15 minutes at R.T. by end point method. infinite Liquid STAT Glucose is linear up to 500 mg%. infinite Liquid STAT Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request. Sodium Fluoride (as an anticoagulant up to 10 mg/ml blood) does not effect glucose assay. The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Haemoglobin is negligible. COMPONENTS & CONCENTRATION OF WORKING SOLUTION Component Concentration Phosphate Buffer, pH 7.0 170mmol/I Glucose oxidase 15000IU/I Peroxidase 1500 IU/I 4 - amino antipyrine 0.28 mmol/I Phenol 16 mmol/I Stabilizers and inactive ingredients

Glucose Eco-Pak 500

INTRODUCTION Eco-Pak Glucose is a reagent set for determination of True Glucose, based on enzymatic method using Glucose oxidase and Peroxidase. Eco-Pak Glucose has one step reconstitution. It involves the mixing of Enzyme and Diluent. Eco-Pak Glucose is linear up to 450 mg%. Eco-Pak Glucose is a High Stability Reagent. Eco-Pak Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. Eco-Pak Glucose can be determined in just 15 minutes at 37 degree C or 30 minutes at R.T. (25-30 degree C). Sodium Fluoride (as an anticoagulant up to 10 mg/ml blood) does not affect glucose assay. The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible. The reagent can also determine glucose using whole blood (0.02 ml) collected from finger-prick. The procedure is available upon request.

Diluent Glucose Eco-Pak 500

INTRODUCTION Eco-Pak Glucose is a reagent set for determination of True Glucose, based on enzymatic method using Glucose oxidase and Peroxidase. Eco-Pak Glucose has one step reconstitution. It involves the mixing of Enzyme and Diluent. Eco-Pak Glucose is linear up to 450 mg%. Eco-Pak Glucose is a High Stability Reagent. Eco-Pak Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. Eco-Pak Glucose can be determined in just 15 minutes at 37 degree C or 30 minutes at R.T. (25-30 degree C). Sodium Fluoride (as an anticoagulant up to 10 mg/ml blood) does not affect glucose assay. The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible. The reagent can also determine glucose using whole blood (0.02 ml) collected from finger-prick. The procedure is available upon request. PREPARATION OF WORKING SOLUTION Reconstitute enzyme & diluent as per instruction indicated on individual bottle label to prepare working solution. Mix by gentle swirling or inversion. DO NOT SHAKE VIGOROUSLY.

HDL Cholestrol 100

brand name : ACCUREX BIOMEDICAL

Autozyme Calcium 200

INTRODUCTION Auto Zyme Calcium is a reagent set for determination of calcium based on the colorimetric method using o-Cresol phthalein Complex one. Auto Zyme Calcium is a single reagent system, using one step procedure. Auto Zyme Calcium is linear u pto 15 mg% Auto Zyme Calcium is a High Stability Reagent. Auto Zyme Calcium can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. Auto Zyme Calcium has one step reconstitution. It involves mixing of CPC and Diluent reagent. Calcium can be determined in just 5 minutes. Auto Zyme Calcium has minimum reagent wastage since working solution can be prepared according to the need. Interference of Mg and Fe is negligible. Lipemic samples do not require any special treatment.

Total Protein 300

INTRODUCTION Auto Zyme Total protein is a reagent set for determination of total protein based on Biuret method. Auto Zyme Total protein is a single reagent system, ready-to-use. Auto Zyme Total protein is linear up to 18 gm%. Total protein can be determined in just 5 minutes. Auto Zyme Total protein does not require serum blank for lipemic samples. Auto Zyme Total protein can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request. The shelf-life of Auto Zyme Total protein is 18 months. PREPARATION OF WORKING SOLUTION The reagent is ready-to-use. REAGENT STORAGE & STABILITY The reagents are for in vitro diagnostic use. Biuret reagent & standard should be stored at temperature indicated on the bottle label.