Price: 2036 INR/Kit
INTRODUCTION
Autozyme Glucose is a reagent set for determination of True Glucose, based on enzymatic method; using Glucose, oxidase and Peroxidase.
Autozyme Glucose is a ready-to-use reagent.
AutozymeGlucose estimates glucose in 7 minutes at 37 Degree C or15 minutes at R.T. by end point method.
Autozyme Glucose is linear up to 500 mg%.
Autozyme Glucose can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
Autozyme (as an anticoagulant up to 10 mg/ml blood) does not effect glucose assay.
The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Haemoglobin is negligible.
PRINCIPLE
Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of Peroxidase (POD), oxidatively couples with 4-aminoantipyrine and phenol to produce red quinoneimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.). The intensity of the colour complex is directly proportional to the concentration of glucose in specimen.