Bilirunin (T And D) 200

Bilirunin (T And D) 200

Product Details:

  • Supply Ability : 10000 Kit Per Day
1283 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 1283 INR/Kit

Trade Information

  • EXW ABPL
  • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION
  • Auto zyme Bilirubin is a reagent set for determination of Total and Direct Bilirubin based on Jurassic & Grof method, using Diazotized Sulphonic Acid with DMSO as an activator for Total Bilirubin.
  • Auto Zyme Bilirubin is a two reagent system using one step procedure.
  • Auto Zyme Bilirubin is linear upto 20 mg%.
  • Auto Zyme Bilirubin can be used on any Colorimeter, Spectrophotometer, Discrete semi-automated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
  • Auto Zyme Bilirubin has one step reconstitution.
  • Auto Zyme Bilirubin can be determined in just 5 minutes.
  • Auto Zyme Bilirubin method is relatively free of interference from hemoglobin and other commonly occurring substances in serum or plasma.
  • This insert covers technical matter for the complete range of Auto Zyme Bilirubin kits available viz.:

Product

Pack-Size

Bilirubin T & D (Total and Direct) 200

1 x 200 ml

Bilirubin Total 1000

1 x 1000 ml

Bilirubin Direct 1000

1 x 1000 ml

Please read the appropriate matter depending on the kit you have purchased.

PRINCIPLE

Bilirubin reacts with diazotized sulphanilic acid to produce azobilirubin (violet colour). DMSO catalyzes the formation of azobilirubin from free bilirubin. The violet colour is proportional to bilirubin concentration measured at 546 nm.(530 - 550 nm.).

TOTAL BILIRUBIN

Bilirubin + Sulphanilic Acid + Sod. Nitrite DMSO x p Azobilirubin

DIRECT BILIRUBIN

Bilirubin + Sulphanilic Acid + Sod. Nitrite .Azobilirubin

DMSO  Dimethyl Sulphoxide

REAGENT STORAGE & STABILITY

  • The reagent kit should be stored at Room Temperature (25-30 degree C) and is stable till the expiry date indicated on the label.
  • The working solution is stable for 2 days at 2-8 degree C and for 5 hours at Room Temperature (25-30 degree C).
COMPONENTS & CONCENTRATION OF WORKING SOLUTION

Component

Concentration

 

Total Bilirubin

Direct Bilirubin

Sulphanilic Acid

32 mmo1/1

32 mmo1/1

Sodium Nitrite

290 mmo1/1

29 mmo1/1

Hydrochloric Acid

165 mmo1/1

165 mmo1/1

DMSO

7 mmo1/1

 

 SPECIMEN COLLECTION & PRESERVATION

Blood should be collected in a clean dry container. Although serum is preferred plasma can also be used as sample.

Following anticoagulant can be used for plasma separation :

  • EDTA 2 mg/ml of blood
  • HEPARIN 200 IU/ml of blood

Bilirubin is light sensitive. Avoid exposure of serum or plasma to direct light. Bilirubin in serum and plasma is stable for a day at 2-8 degree C or one month at -10 degree C. Samples should be brought to room temperature before use.

PROCEDURE

  • Reaction type: End-Point
  • Reaction time: 5 mins. at R.T. (25-30 degree C)
  • Wavelength: 546 nm.(530 - 550 nm.)
  • Zero setting with : Serum Blank
  • Sample volume: 0.05 ml (50 III)
  • Reagent volume: 1.02 ml
  • Factor: 20.2
  • Linearity: 20 mg%
Manual assay procedure

Perform the assay as given below :

Serum Blank


Test

Reagent T1

 1.0 ml

1.0 ml

Reagent T2

 

0.02 ml

Serum / Plasma

0.05 ml

0.05 ml

 DIRECT BILIRUBIN

Serum Blank

 

Test

Reagent T1

1.0 ml

1.0 ml

Reagent T2

 

0.02 ml

Serum / Plasma

0.05 ml

0.05 ml

 Mix, and incubate the assay mixture at Room Temperature (25-30 degree C) for 5 minutes. Read before 8 minutes the absorbance of the test against their respective blanks at 546 nm (530 - 550 nm).

EXPECTED VALUES

 

  • Total Bilirubin: up to 1.0 mg%
  • Direct Bilirubin: up to 0.3 mg%

 

PROCEDURE LIMITATIONS

Dilute the specimen if the Bilirubin value is above 20 mg%. Suitable dilution can be done with normal saline. In such case the results obtained should be multiplied by dilution factor to obtain correct Bilirubin value.

QUALITY CONTROL

It is recommended that each batch should include a normal and an abnormal commercial reference control serum or a known patient serum. Use of quality control serum, checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware, accuracy of pipetting and serum exposure to light.

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