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BUN 100
1046 INR/Kit
Product Details:
- Physical Form Liquid
- Appearance Biochemistry reagent
- Usage Infinite Liquid BUN is aready-to-use, two liquid reagent system. Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute. Infinite Liquid BUN is linear upto 250 mg/dl.
- Shelf Life 18 Months
- Shape other
- Smell Other
- Taste Other
- Supply Ability : 10000 Kit Per Day
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Price And Quantity
- 1 Kit
- 1046 INR/Kit
Product Specifications
- Reagents
- Biochemistry reagent
- Liquid
- 18 Months
- Other
- ACC77889
- Infinite Liquid BUN is aready-to-use, two liquid reagent system. Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute. Infinite Liquid BUN is linear upto 250 mg/dl.
- Other
- other
- Biochemistry reagent
- 100
- Biochemistry reagent
- Other
Trade Information
- EXW ABPL
- Letter of Credit (L/C), Paypal, Letter of Credit at Sight (Sight L/C), Cash Against Delivery (CAD), Cash in Advance (CID), Cheque
- 10000 Kit Per Day
- 2 Days
- Yes
- packaging : Empity
- All India
- certification : iso
Product Description
- Infinite Liquid BUN is a reagent set for determination of blood urea nitrogen (BUN) concentration in serum or plasma based on Enzymatic UV- Kinetic initial rate method.
- Infinite Liquid BUN is aready-to-use, two liquid reagent system.
- Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute.
- Infinite Liquid BUN is linear upto 250 mg/dl.
- Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
- finite Liquid BUN is stable till expiry at 2 - 8C.
PRINCIPLE
Urea is hydrolysed to ammonia and carbondioxide by urease. Ammonia produced reacts with a-ketoglutarate to form glutamate in presence of glutamate dehydrogenase. NADH is oxidised to NAD in this reaction, which is measured as decrease in absorbance at 340 nm. The rate of decrease in absorbance at 340 nm. is directly proportional to BUN concentration in the specimen.
Urea + H,O- 2nH, + CO,
GLDH
NH, + a-ketoglutarate + NADH -glutamate + NAD
Abbreviations
GLDH - Glutamate dehydrogenase
REAGENT STORAGE & STABILITY
- The reagent kit should be stored at 2 - 8 C and is stable till the expiry date indicated on the label.
- R, and R, reagents are stable till expiry at 2 - 8C.
- The working solution (4 R, + 1 R,) is stable for 30 days at 2 - 8 C.
COMPONENTS & CONCENTRATION OF WORKING SOLUTION
|
Component |
Concentration |
|
Tris buffer, pH 7.6 |
120 mmol/l |
|
Urease |
20 KU/l |
|
Glutamate dehydrogenase |
1 KU/I |
|
NADH |
0.25 mmoll/| |
|
ketoglutarate |
10 mmol/l |
PROCEDURE
- Reaction type : UV-Kinetic
- Reaction direction: Decreasing
- Wavelength : 340 nm
- Flowcell temperature: .25 Degree C/30 Degree C
- Zero setting with: Distilled water
- Delay time: 300 seconds
- No. of readings: 2
- Interval : 30 seconds
- Sample volume: 1.000 Abs
- R 1 volume: 360 Ul
- R 2 volume: 180 Ul
- Factor: 1743
- Linearity: 250mg /dl
MANUAL ASSAY PROCEDURE .
- Prewarm at 25Degree C / 30 Degree C the required amount of working solution before use. Perform the assay as given below : Working solution: 1.0 mi (800 U1 R, + 200 LI R,) Mix and aspirate. After the initial delay of 30 seconds, record the absorbance of the test at an interval of 30 seconds at 340 nm.
- Determine the change in absorbance ( A Abs.) and calculate test result.
- Serum / Plasma
Expected Values
- Urea Nitrogen (BUN) : 5-21 mg/dl
- Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.
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Contact Details
ACCUREX BIOMEDICAL PVT. LTD.
- 212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai - 400016, Maharashtra, India
- Phone : 08045479256
- Fax : 91-22-66662223
- Mobile : 07710092831
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