BUN 500 - Infinite

BUN 500 - Infinite

Product Details:

  • Physical Form Other
  • Smell Other
  • Usage Infinite Liquid BUN is linear upto 250 mg/dl. Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
  • Taste Other
  • Appearance Biochemistry reagent
  • Other Names Biochemistry reagent
  • Shape other
  • Supply Ability : 10000 Kit Per Day
6043 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 6043 INR/Kit

Product Specifications

  • ACC226985
  • Biochemistry reagent
  • Biochemistry reagent
  • other
  • Other
  • 18 Months
  • Reagents
  • Other
  • Other
  • Infinite Liquid BUN is linear upto 250 mg/dl. Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
  • 100
  • Biochemistry reagent
  • Other

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

  • Infinite Liquid BUN is a reagent set for determination of blood urea nitrogen (BUN) concentration in serum or plasma based on Enzymatic UV- Kinetic initial rate method.
  • Infinite Liquid BUN is aready-to-use, two liquid reagent system.
  • Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute.
  • Infinite Liquid BUN is linear upto 250 mg/dl. 
  • Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
  • finite Liquid BUN is stable till expiry at 2 - 8C.
PRINCIPLE

Urea is hydrolysed to ammonia and carbondioxide by urease. Ammonia produced reacts with a-ketoglutarate to form glutamate in presence of glutamate dehydrogenase. NADH is oxidised to NAD in this reaction, which is measured as decrease in absorbance at 340 nm. The rate of decrease in absorbance at 340 nm. is directly proportional to BUN concentration in the specimen. 

Urea + H,O- 2nH, + CO,

GLDH

NH, + a-ketoglutarate + NADH -glutamate + NAD

Abbreviations

GLDH - Glutamate dehydrogenase 

REAGENT STORAGE & STABILITY

  • The reagent kit should be stored at 2 - 8 C and is stable till the expiry date indicated on the label. 
  • R, and R, reagents are stable till expiry at 2 - 8C.
  • The working solution (4 R, + 1 R,) is stable for 30 days at 2 - 8 C. 

COMPONENTS & CONCENTRATION OF WORKING SOLUTION

Component

Concentration

Tris buffer, pH 7.6

120 mmol/l

Urease

20 KU/l

Glutamate dehydrogenase

1 KU/I

NADH

0.25 mmoll/|

ketoglutarate

10 mmol/l

 PROCEDURE

  • Reaction type :  UV-Kinetic 
  • Reaction direction:  Decreasing 
  • Wavelength :  340 nm 
  • Flowcell temperature:  .25 Degree C/30 Degree C
  • Zero setting with:  Distilled water 
  • Delay time:  300 seconds 
  • No. of readings:   2 
  • Interval :   30 seconds 
  • Sample volume:  1.000 Abs
  • R 1 volume:   360 Ul 
  • R 2 volume:  180 Ul 
  • Factor:   1743 
  • Linearity:   250mg /dl
MANUAL ASSAY PROCEDURE .

Prewarm at 25Degree  C / 30 Degree  C the required amount of working solution before use. Perform the assay as given below : Working solution: 1.0 mi (800 U1 R, + 200 LI R,) Mix and aspirate. After the initial delay of 30 seconds, record the absorbance of the test at an interval of 30 seconds at 340 nm.

Determine the change in absorbance ( A Abs.) and calculate test result. 

Serum / Plasma

Expected Values 

Urea Nitrogen (BUN) : 5-21 mg/dl

Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. 

Enter Buying Requirement Details
Email Id
Mobile number

Other Products in 'Infinite Reagents' category



Back to top
ACCUREX BIOMEDICAL PVT. LTD. All Rights Reserved.(Terms of Use)
Developed and Managed by Infocom Network Private Limited.