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Reagent Storage, Stability & Handling
AutoPure Chloride is a ready to use single liquid reagent system.
Stable till the expiry date indicated on the label, when stored at15 - 25 C.
On Board Reagent Stability
45 days at 2 -8 C after opening. Protect the reagent from light and contamination.
Do not freeze the reagent.
Components & Concentration of Working Solution
Specimen collection & Preservation
Collect sample using standard sampling tube. Serum or heparinized plasma can be used. Grossly haemolyzed serum should not be used as it may give false low results. Chloride in serum/ plasma is stable for 7 days at 2- 8 C and 30 days at -20 C. Centrifuge samples containing pricipate before performing assay.
Refer to the application sheet for details. For additional system applications, contact our local accurex representative.
Re- calibration is recommended
Each batch of autopure Chloride is assayed with atleast six quality control sera- Precinorm, precipath, bioradl, bioradll, accutrol normal and Accutrol abnormal prior to release.
To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commerical reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.
If the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:
Expected range varies from population to population. it is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the bilirubin results should always be assessed in diagnostic purposes, the bilirubin results should always be assessed in conjuction with the patients medical history clinical examinations and other findings.
With Autopure Chloride , the assay is linear up to 130 mg/dl (458.9 mmol/l) .Determine samples with higher concentration via the rerun function . On instrument without rerun function, manually dilute samples with higher concentration using or distilled/deionized water (e.g. 1+2 ). Multiply the result by the appropriate dilution factor (e.g. 3).
There is no significant interference in samples containing upto 30 mg/dl of bilirubin and 300 mg/dl of haemoglobin.
Reproducibility was determined using quality control sera as shown below
Quality control material
low control serum
high control serum
Co- Relation Studies
A comparison of the Chloride determination using autopure Chloride Autozyme chloride reagent gave the following co- realtion (mmol/l)
No of samples measured : 72
The samples concentrations measured were between 80 mmol/l and 120 mmol/l.