Chloride 80

Chloride 80

Product Details:

  • Taste Other
  • Smell Other
  • Shape other
  • Other Names Biochemistry Reagent
  • Physical Form Liquid
  • Shelf Life 18 Months
  • Appearance Biochemistry Reagent
  • Supply Ability : 10000 Kit Per Day
2403 INR/Kit

Price And Quantity

  • 2403 INR/Kit
  • 1 Kit

Product Specifications

  • other
  • Other
  • Reagents
  • Other
  • AutoPure Chloride is ready to use , single liquid reagent system. With AutoPure Chloride, the assay is linear upto 130 mg/dl( 458.9 mmol/l).
  • Biochemistry Reagent
  • 100
  • Biochemistry Reagent
  • 18 Months
  • Liquid
  • ACC9053
  • Biochemistry Reagent
  • Other

Trade Information

  • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • certification : iso

Product Description


  •  AutoPure Chloride  is a reagent kit for direct quantitative determination of  Chloride in human serum and plasma on automated clinical chemistry analyzers.
  •   AutoPure Chloride is ready to use , single  liquid reagent system.
  • With   AutoPure Chloride, the assay is linear upto  130 mg/dl( 458.9 mmol/l).


  • Chloride ions react with mercuric thiocyanate to form undissociated mercuric chloride and free thiocyanate ions.
  • Hg (SCN)2 + 2 Cl- hgcl2 + 2SCN
  • Thiocyanate ions react with trivalent ferric ions present in the solution to form a red coloured complex of ferric thiocyanate.
  • 3 (SCN)+ Fe3 - Fe( SCN) 3 
  • The intensity of the red colour formed is directly proportional to the concentration of chloride in the specimen and is masured photometrically .

Reagent Storage, Stability & Handling

AutoPure  Chloride is a ready to use single liquid reagent system.  

Shelf life  

Stable till the expiry date indicated on the label, when stored at15 - 25 C. 

On Board Reagent Stability

45 days at 2 -8 C after opening. Protect the reagent from light and contamination.

Do not freeze the reagent. 

Components & Concentration of Working Solution  

Components                                                                                Concentration

  • Mercuric thiocyanate                                                              2.0 mmol/l
  •  Mercuric Chloride                                                                   1.0 mmol/l
  • Ferric nitrate                                                                              20 mmol/l
  • Nitric acid                                                                                 45mmol/l 
  • Stabilizers, excipients & surface active agents 

Specimen collection & Preservation

Collect sample using standard sampling tube. Serum or heparinized  plasma can be used. Grossly haemolyzed serum should not be  used as it may give false low results. Chloride in serum/ plasma is stable for 7 days at 2- 8 C and 30 days at -20 C. Centrifuge samples containing pricipate before performing assay.


  • Fully automated system automatically calculated the Chloride   concentration of each samples.
  •  Result in mmol/dl =  Result in mg/l x 3.53

Application Sheet 

Refer to the application sheet for details. For additional system applications, contact our local accurex representative.


Re- calibration is recommended 

  • Whenever the reagent lot is changed .
  • As per the requirement of quality control procedured.

Quality Control

Each batch of autopure Chloride  is assayed with atleast six quality control sera- Precinorm, precipath, bioradl, bioradll, accutrol normal and Accutrol abnormal prior to release.

To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commerical reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

If the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:

  • Proper instruments function- wavelength setting, light source and temperature control.
  • Cleanliness of probes and cuvettes.
  • Bacterial contamination of wash water used by the instrument.
  • Expiry date of the reagent kit. 

Expected Values 

  • Serum/Plasma
  • 98- 107 mmol/l( 346-377mg/dl)


Expected range varies from population to population. it is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the bilirubin results should always be assessed in diagnostic purposes, the bilirubin results should always be assessed in conjuction with the patients medical history clinical examinations and other findings.

Performance Characteristics 


With Autopure  Chloride   , the assay is linear up to 130 mg/dl (458.9 mmol/l) .Determine samples with higher concentration via the rerun function . On instrument without rerun function, manually dilute samples with higher concentration using or distilled/deionized water (e.g. 1+2 ). Multiply the result by the appropriate dilution factor (e.g. 3). 


 There is no significant interference in samples containing upto 30 mg/dl of bilirubin and 300 mg/dl of haemoglobin.


Reproducibility was determined using quality control sera as shown below 


Within run

Between run

Quality control material

Means mmol/l

sd mmol/l


Means mmol/l

sd mmol/l


low control serum

87.6 2.32 2.7 89.4 1.12


high control serum

107.0 1.06 1.0 109.0



  Co- Relation Studies

A comparison of the Chloride   determination using autopure  Chloride   Autozyme chloride   reagent gave the following co- realtion (mmol/l)

Linear Regression

  • y=                             0.987 x + 10.593
  • r=                             1.001
  • Sy.x=                      1.46

No of samples measured : 72 

The samples concentrations measured were between 80 mmol/l and 120 mmol/l.


  • Burtis C.A. Ashwood E.R eds. Tietz textbook of clinical  chemistry ,3rd ed. Philadelphia pa : w.b saunders 1994: 1368
  • Zall D. Fisher d. and Gaarner m . Anal. chem 28: 1665, 1956.
  • Schoenfeld R.G and lerveller C.v clin chem 10,533, 1964. 
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