Cholesterol 50

Cholesterol 50

Product Details:

  • Physical Form Liquid
  • Usage Infinite Cholesterol can be determined in just 5 minutes at 37 Degree C or 10 minutes at R.T. (25 -30 Degree C). Infinite Liquid Cholesterol is linear upto 1000 mg%. Infinite Liquid Cholesterol can be used on any Colorimeter Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
  • Other Names Biochemistry reagent
  • Shape other
  • Appearance Biochemistry reagent
  • Taste Other
  • Shelf Life 18 Months
  • Supply Ability : 1 Kit Per Day
1046 INR/Kit
X

Price And Quantity

  • 1046 INR/Kit
  • 1 Kit

Product Specifications

  • Biochemistry reagent
  • Other
  • Infinite Cholesterol can be determined in just 5 minutes at 37 Degree C or 10 minutes at R.T. (25 -30 Degree C). Infinite Liquid Cholesterol is linear upto 1000 mg%. Infinite Liquid Cholesterol can be used on any Colorimeter Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
  • other
  • Biochemistry reagent
  • Biochemistry reagent
  • Liquid
  • Reagents
  • 18 Months
  • ACC744747
  • Other
  • 100
  • Other

Trade Information

  • Cash Against Delivery (CAD), Cash on Delivery (COD), Cash Advance (CA), Cash in Advance (CID), Cheque, Paypal
  • 1 Kit Per Day
  • 1 Week
  • All India

Product Description

  • Infinite Liquid Cholesterol is a reagent set for determination of total cholesterol based on enzymatic method using Cholesterol esterase, Cholesterol oxidase and Peroxidase.
  • Infinite Liquid Cholesterol is a ready -to-use reagent.
  • Infinite Cholesterol can be determined in just 5 minutes at 37 Degree C or 10 minutes at R.T. (25 -30 Degree C).
  • Infinite Liquid Cholesterol is linear upto 1000 mg%.
  • Infinite Liquid Cholesterol can be used on any Colorimeter Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
  • The influence of Ascorbic acid, Bilirubin, Haemoglobin, Glucose, Sodium fluoride, Heparin, EDTA, Creatinine and Uric acid is negligible.
  • Infinite Liquid Cholesterol reagent system can also be used for determining HDL Cholesterol. The HDL ppt reagent and standard can be ordered separately.
PRINCIPLE

Cholesterol esterase hydrolyses cholesterol esters into free cholesterol and fatty acids. In the second reaction cholesterol oxidase converts cholesterol to cholest-4-en-3-one and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with 4 - aminoantipyrine and phenol to produce red quinoneimine dye which has absorbance maximum at 510 nm. (500 - 530). The intensity of the red colour is proportional to the amount of total cholesterol in the specimen.

Abbreviations

  • CHE - Cholesterol esterase
  • CHO - Cholesterol oxidase
  • POD - Peroxidase 
REAGENT STORAGE & STABILITY & HANDLING
  • The kit should be stored at 2- 8 Degree C and is stable till the expiry date indicated on the label.
  • The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8 Degree C. DO NOT FREEZE THE REAGENT.
  • The reagent should be stored only in the amber bottle provided to protect it from direct light. Before use swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
  • Over time, the reagent may develop a light pink colour. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300 O.D. against distilled water at 510 nm.
  • Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity discard the reagent.
COMPONENTS & CONCENTRATION OF WORKING SOLUTION

Component

Concentration

Buffer, pH 6.8

50 mmol/l

Cholesterol oxidase

 100  IU/I

Cholesterol esterase

150  IU/I

Peroxidase

500 IU/I

4- aminoantipyrine

0.5 mmol/|

Phenol

10 mmol/|

Stabilizers / Surfactants

 

SPECIMEN COLLECTION & PRESERVATION

Blood should be collected in a clean dry container. Fasting blood is preferred for cholesterol assay. Cholesterol in the serum is stable for 7 days when stored at 2-8 Degree C and 60 days when stored at -20 Degree C.

PROCEDURE

  • Reaction type: End-Point
  • Reaction time: .5 mins. at 37 Degree C/10 mins. at R.T. (25-30 Degree C)
  • Wavelength :  510 nm. (500 - 530 nm.)
  • Zero Setting WIN : REAGENT Blank
  • Blank absorbance limit: 0.300 Abs.
  • Sample VOIUME:  0.01 mi (10 UI)
  • Reagent Volume: 1.0 MI
  • Standard concentration : 200 Mg%
  • Linearity: 1000 mg/dl 
Manual assay procedure

Prewarm at room temperature the required amount of reagent before use.

Perform the assay as given below :

1.0 ml procedure

 

Serum

Standard

Blank

Reagent

0.01ml

0.01ml

-

1.0ml

1.0ml

1.0ml

 Incubation

Incubate the assay mixture for 5 minutes at 37 Degree C or 10 minutes at room temperature (25-30 Degree C). After incubation measure the absorbance of assay mixture against blank at 510 nm. The final colour is stable for two hours if not exposed to direct light.

EXPECTED VALUES 

Desirable Cholesterol

 200 mg/dl

Borderline High Cholesterol

200- 239 mg/dl

High Cholesterol

240 mg/dl

 Expected range varies from population to population. It is recommended that each laboratory should establish its own normal range.

Enter Buying Requirement Details
Email Id
Mobile number

Other Products in 'Infinite Reagents' category



Back to top
ACCUREX BIOMEDICAL PVT. LTD. All Rights Reserved.(Terms of Use)
Developed and Managed by Infocom Network Private Limited.