Cholinesterase 18

Cholinesterase 18

Product Details:

  • Shelf Life 18 Months
  • Smell Other
  • Taste Other
  • Other Names Biochemistry reagent
  • Physical Form Liquid
  • Appearance Biochemistry reagent
  • Usage Infinite Cholinesterase is a ready-to-use, two liquid reagent system. With Infinite Cholinesterase, the assay is linear upto 25000 U/L.
  • Supply Ability : 10000 Kit Per Day
1236 INR/Kit
X

Price And Quantity

  • 1236 INR/Kit
  • 1 Kit

Product Specifications

  • Other
  • Reagents
  • Other
  • Liquid
  • Biochemistry reagent
  • Biochemistry reagent
  • Infinite Cholinesterase is a ready-to-use, two liquid reagent system. With Infinite Cholinesterase, the assay is linear upto 25000 U/L.
  • Other
  • other
  • Biochemistry reagent
  • 18 Months
  • 100
  • ACC66969

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION 
  • Infinite Cholinesterase is a reagent kit for quantitative  determination of cholinesterase activity in human serum and  plasma based on DGKC method using butyrylthiocholine. 
  • Infinite Cholinesterase is a ready-to-use, two liquid reagent system.
  • With Infinite Cholinesterase, the assay is linear upto 25000 U/L.
  • Infinite Cholinesterase assay can be performed in 8 minutes at  37 Degree C. 
CLINICAL SIGNIFICANCE 

Cholinesterase also known as acetylcholinesterase, is found mainly in  the nerve endings and in the gray matter of the brain. It is responsible  for prompt hydrolysis of acetylcholine released at the nerve endings to  mediate transmission of the neural impulse across the synapse. The  degradation of the acetylcholine is necessary to the depolarization of  the nerve so that it can repolarized in the next conduction event. 

A similar enzyme acylcholine acylhydrolase, also known as  pseudocholinesterase, is found in the liver, heart, pancrease, white  matter of the brain and serum, but its biological role is unknown. 

 Cholinesterase levels in serum are useful as a test of liver function and as an indicator of possible insecticide poisoning. Among the organic phosphorus compounds that inhibit the cholinesterase activity are  many organic insecticides, such as Parathion, Sarin and tetraethyl pyrophosphate. During poisoning the level of enzyme decreases as its activity is inhibited.

REAGENT STORAGE,  STABILITY & HANDLING 

  • The reagents R1 & R2 are ready-to-use. 
  • The reagent kit should be stored at 2 Degree C  - 8 Degree C and is stable till the expiry  date indicated on the label. 
  • Keep the reagents away from direct light sources. Do not freeze the  reagent. 
  • Contamination of reagent should be strictly avoided 

COMPONENTS & CONCENTRATION OF WORKING SOLUTION

Component

Concentration

Sodium pyrophosphate

 70 mM

Potassium ferricyanide

 1 mM

 Butyrylthiocholine

12 mM

SPECIMEN COLLECTION & PRESERVATION

Blood should be collected in a clean dry container. Serum or EDTA/heparinized plasma can be used. Hemolysis should be avoided. Cholinesterase activity in serum/plasma is stable for 2 week 2 Degree C - 8 Degree C.

PROCEDURE

  • Reaction type: .Kinetic 
  • Reaction direction: Decreasing 
  • Wavelength: 405 nm 
  • Flowcell temperature: 37 Degree C
  • Zero setting with: .Distilled water 
  • Delay time: 90 seconds 
  • No. of readings: 4 
  • Interval: 30 seconds 
  • Sample volume: 20 ul 
  • R 1 volume: 1000 ul 
  • R 2 volume: 200 ul 
  • Factor: 65800 
  • Linearity:  25000 U/L  
Manual assay procedure : 

Perform the assay as given below: 
  • R 1 : 1.0 ml (1000 ul) 
  • Sample:  0.020 ml (20 ul) 
Mix & incubate for 5 minutes at 37 Degree C 

R2 :  0.200 ml (200 ul) 
  • Mix & aspirate the assay mixture. After the initial delay  of 90 seconds, record the absorbance of the test at an  interval of 30 seconds for the next 90 seconds at 405  nm. 
  • Determine the mean change in absorbance per minute  and calculate the test results. 
Calculation : 

Activity of Cholinesterase in U/L -Abs/min. X 65800

EXPECTED VALUES

  • Men : 5600 - 11200 U/L 
  • Women : 4200 - 10800 U/L 

Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own reference   range. 

PERFORMANCE CHARACTERISTICS

  •  Linearity limit: If the Cholinesterase activity exceeds 25000 U/L, dilute the specimen with normal saline (0.9% NaCl) and repeat  the assay. The result obtained should be then multiplied with  dilution factor to obtain correct Cholinesterase activity. 
  • Detection Limit: Values less than 432.3 U/L gives non reproducible results. 
  • Interferences: No interference was observed upto 50 
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