Price: 5943 INR/Kit
Infinite ADA is a reagent set for direct quantitative determination of Adenosine deaminase (ADA) activity in human serum, plasma and other body fluids.
Infinite ADA is a ready-to-use, two liquid reagent system.
With Infinite ADA, the assay is linear upto 200 U/L.
Infinite ADA assay can be performed in 11 minutes at 37 C.
ADA catalyses deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleosidephosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4 AAP) in the presence of peroxidase (POD) to generate quinone dye.
denosine + H2O Inosine + NH3 PNP Inosine + Pi Hypoxanthine + Ribose-1-phosphate XOD Hypoxanthine + 2H2O + 2O2 Uric acid + 2 H2O2 POD 2 H2O2 + 4AAP + EHSPT 4 H2O + Quinone dye
The intensity of the colour developed is directly proportional to the activity of ADA in the specimen and is measured kinetically.