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Note : Over a period of time, the reagent R2 after reconstitution may develop a yellow colour. This is expected and does not affect the performance of the assay.
REAGENT STORAGE & STABILITY
COMPONENTS & CONCENTRATION OF WORKING SOLUTION
R1 Buffer Reagent
Glycylglycine, pH 8.5: 170 mmo1/1
L-y-glutamyl-3-carboxy-4-nitroanilide: 3 mmol/1
R2(b) Diluent for Substrate
MES buffer, pH 5.5: 4 mmo1/1
SPECIMEN COLLECTION & PRESERVATION
Blood should be collected in a clean dry container. Although serum is preferred, plasma with heparin or EDTA can be used. GGT in serum/plasma is stable for 7 days at 2 - 8 degree C and 6 months at -20 degree C. Centrifuge samples containing precipitate before performing the assay. The samples should be brought to room temperature prior to use.
Perform the assay as given below:
1.0 ml procedure
Mix to the thermostated cuvette and start the stop watch simultaneously. Record the first reading at 60th second and subsequently two more readings with 60 seconds interval at 405 nm.
at 37 Degree C
8 - 61 IU/1
5 - 36 IU/1
Expected range varies from population to population. It is therefore
recommended that each laboratory should establish its own normal range
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control the serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.