Gamma GT 10

Gamma GT 10

Product Details:

  • Physical Form Liquid
  • Taste Other
  • Shelf Life 18 Months
  • Smell Other
  • Appearance Biochemistry reagent
  • Shape other
  • Other Names Biochemistry reagent
  • Supply Ability : 10000 Kit Per Day
1285 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 1285 INR/Kit

Product Specifications

  • Auto Zyme Gamma-GT is a ready-to-use, two liquid reagent system, using single step reconstitution. Auto Zyme Gamma-GT estimates GGT activity in just 3 minutes.
  • Reagents
  • Biochemistry reagent
  • Biochemistry reagent
  • other
  • Other
  • 100
  • ACC333633
  • Biochemistry reagent
  • 18 Months
  • Other
  • Other
  • Liquid

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION
  • Auto Zyme Gamma-GT is a reagent set for determination of gamma glutamyl transferase (GGT) in serum and plasma based on IFCC method.
  • Auto Zyme Gamma-GT is a ready-to-use, two liquid reagent system, using single step reconstitution.
  • Auto Zyme Gamma-GT estimates GGT activity in just 3 minutes.
  • Auto Zyme Gamma-GT is linear up to 1000 IU/1.
  • Auto Zyme Gamma-GT can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. Programmed can be designed for any specific analyzer upon request.
  • Auto Zyme Gamma-GT is stable till expiry at 2- 8 degree C.
PRINCIPLE

Gamma-glutamyl transferase (GGT) transfers the y-glutamyl group of L-y-glutamyl-3-carboxy-4-nitroanilide to glycylglycine. The amount of 5-amino-2-nitrobenzoate liberated at 405 nm. is proportional to the activity of GGT in serum/plasma and is measured kinetically.

PREPARATION OF  WORKING SOLUTION
  • R1 : Buffer reagent is ready-to-use.
  • R2 : Transfer contents of diluent bottle R2(b) into substrate bottle R2(a) as per the instructions indicated on the label. Mix gently by inversion. Prepare working solution by mixing Reagent R1 and Reagent R2 in the ratio 4 : 1 as per requirement.

Note : Over a period of time, the reagent R2 after reconstitution may develop a yellow colour. This is expected and does not affect the performance of the assay.

REAGENT STORAGE & STABILITY

  • The reagent kit should be stored at 2 - 8 degree  C and is stable till the expiry date indicated on the label.
  • R1 & R2 reagents are stable till expiry at 2 - 8 degree  C.
  • The working solution (4 R1 + 1 R2) is stable for 21 days at 2 - 8 degree C.

COMPONENTS & CONCENTRATION  OF WORKING SOLUTION

R1 Buffer Reagent

Glycylglycine, pH 8.5: 170 mmo1/1 

R2(a) Substrate

L-y-glutamyl-3-carboxy-4-nitroanilide: 3 mmol/1 

R2(b) Diluent for Substrate

MES buffer, pH 5.5: 4 mmo1/1

SPECIMEN COLLECTION & PRESERVATION

Blood should be collected in a clean dry container. Although serum is preferred, plasma with heparin or EDTA can be used. GGT in serum/plasma is stable for 7 days at 2 - 8 degree  C and 6 months at -20 degree  C. Centrifuge samples containing precipitate before performing the assay. The samples should be brought to room temperature prior to use.

 PROCEDURE

  • Reaction type: Kinetic
  • Reaction direction : Increasing
  • Wavelength :405 nm.
  • Flowcell temperature: 37 degree C
  • Zero setting with : Working solution
  • Delay time : 60 seconds
  • No. of readings: 3
  • Interval: 60 seconds
  • Sample volume: 0.05 ml (50 gl)
  • Working solution volume (4 Ft, : 1 R2): 1.0 ml (1000 gl)
  • Factor: 2201
  • Linearity: 1000 IU/1
Manual assay procedure

Prewarm at 37 degree C the required amount of working solution before use.

Perform the assay as given below:

1.0 ml procedure

  • Serum / Plasma: 0.05 ml
  • Working solution :  1.0 ml (800 gl R1 + 200 gl R2)

Mix to the thermostated cuvette and start the stop watch simultaneously. Record the first reading at 60th second and subsequently two more readings with 60 seconds interval at 405 nm.

EXPECTED VALUES

Serum/Plasma 

Temperature

at 37 Degree C

Men

8 - 61 IU/1

Women

5 - 36 IU/1

 Expected range varies from population to population. It is therefore 

recommended that each laboratory should establish its own normal range

PROCEDURE LIMITATIONS

  • If the GGT activity exceeds 1000 IU/I, dilute the specimen with normal saline and repeat the assay. In such cases the results obtained should be multiplied with the dilution factor to obtain correct GGT activity.
  • The working solution is considered unsatisfactory and should not be used if the absorbance exceeds 1.000 at 405 nm. against distilled water.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control the serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

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