Gamma-GT 60

Gamma-GT 60

Product Details:

  • Other Names Biochemistry Reagent
  • Shape other
  • Shelf Life 18 Months
  • Appearance Biochemistry Reagent
  • Usage AutoPure Gamma- GT is a reagent kit for direct gamma - glutamyltransferase ( GGT ) in human serum and plasma on automated clinical chemistry analyzers. AutoPure Gamma- GT is ready to use , two liquid reagent system.
  • Physical Form Liquid
  • Smell Other
  • Supply Ability : 10000 Kit Per Day
5673 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 5673 INR/Kit

Product Specifications

  • Other
  • Other
  • Other
  • 100
  • AutoPure Gamma- GT is a reagent kit for direct gamma - glutamyltransferase ( GGT ) in human serum and plasma on automated clinical chemistry analyzers. AutoPure Gamma- GT is ready to use , two liquid reagent system.
  • ACC9052
  • Biochemistry Reagent
  • Liquid
  • Reagents
  • 18 Months
  • other
  • Biochemistry Reagent
  • Biochemistry Reagent

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

Introduction

  •  AutoPure Gamma- GT  is a reagent kit for direct gamma - glutamyltransferase ( GGT ) in human serum and plasma on automated clinical chemistry analyzers.
  •   AutoPure Gamma- GT is ready to use , two  liquid reagent system.
  • With   AutoPureGamma- GT the assay is linear upto  1200 IU/I( 20.04 ukat/l)).

Reagent Storage, Stability & Handling

AutoPureGamma- GT is a ready to use two liquid reagent system.   

Shelf life  

Stable till the expiry date indicated on the label, when stored at 2- 8 C.  

On Board Reagent Stability

  • R1 : 21 days at 2- 8 C after opening. Protect the reagent from light and contamination.
  • R2:  21 days at 2- 8 C after opening. Protect the reagent from light and contamination.

Do not freeze the reagent.

Note : Over a period of time, the reagent R2 may develop a yellow colour. This is expected and does not impair the performance of the assay.

Components & Concentration of Working Solution 

Components                                                                              Concentration

R1

  • Glycylglycine : pH 8.5                                                                 200 mmol/l
  • Stabilizers excipients & surface active agents 

R2

  • MES buffer : pH5.5                                                                            20 mmol/l
  • L-y-glutamyl-3-carboxy-4-nitroanilide                                                >25 mmol/l
  • Stabilizers excipients & surface active agents 

Specimen collection & Preservation

  • Collect sample using standard sampling tube. Although Serum is preferred  heparinized plasma or EDTA   plasma can be used.
  • GGT  in serum/ plasma is stable for 7 days at 2- 8 C and 180days at -20 C. Centrifuge samples containing pricipate before performing assay.

Calculations 

  • Fully automated system automatically calculated the GGT  activity of each samples. 
  • Result in mkat/l = Result in IUI x 0.016 7

Application Sheet 

Application sheet for the popular fully automated are provided along with the kit. For additional system applications, contact our local accurex representative. 

Calibration

 Determine the factor using calibrator.For  calibration it is recommended  to use C.f. a. s or any other suitable calibrator material. 

Calibration

Re- calibration is recommended 

  • Whenever the reagent lot is changed .
  • As per the requirement of quality control procedured.

Quality Control

Each batch of autopure Gamma-GT   is assayed with atleast six quality control sera- Precinorm, precipath, bioradl, bioradll, accutrol normal and Accutrol abnormal prior to release.

To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commerical reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

If the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:

  • Proper instruments function- wavelength setting, light source and temperature control.
  • Cleanliness of probes and cuvettes.
  • Bacterial contamination of wash water used by the instrument.
  • Expiry date of the reagent kit. 

Expected Values 

Serum/Plasma

IU/I

Men

8-61

Women

5-36

ukat/l

Men

0.13-1.02

Women

0.08-0.60

 Note: 

Expected range varies from population to population. it is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the GGT results should always be assessed in diagnostic purposes, the bilirubin results should always be assessed in conjuction with the patients medical history clinical examinations and other findings.

Performance Characteristics  

 Linearity 

With Autopure Gamma-GT  , the assay is linear up to 1200 IU/I (20.04 ukat/l .Determine samples with higher concentration via the rerun function . On instrument without rerun function, manually dilute samples with higher concentration using  0.9 % NaCL or distilled/deionized water (e.g. 1+4 ). Multiply the result by the appropriate dilution factor (e.g. 5).  

Interference  

 There is no significant interference in samples containing upto 30 mg/dl of bilirubin and 90 mg/dl of haemoglobin and 10 mg/dl of ascorbic acid.

Precision

Reproducibility was determined using quality control sera as shown below  

Within run

Between run

Quality control material

Means IU/I

sd IU/I

CV

Means IU/I

sd IU/I

 

CV

 

low control serum

351.363.9371.30

3.5

high control serum

2003.411.7201

201

1.6
1

 Co- Relation Studies

A comparison of theGGT determination using autopure  Gamma- GT assay (y) and reference GGT kit of Daiichl , japan (x) gave the following  co- realtion (IU/I)

Linear Regression

  • y=                          -0.636 + 0.998x
  • r=                             0.9835
  • Sy.x=                      9.62

No of samples measured : 85

The samples concentrations measured were between 10  and 200 IU/I

Reference

  • Persijn, J.P., Van der Slik, W. A new method for the determination of -glutamyltransferase. J. Clin. Chem. Clin. Biochemk. 1976;4:421.
  •  Shaw, L.M., Stromme, J.H., London, J.L., et. al. Clin. Chem. Acta 1983; 135 : 315 - 338.
  •  Shaw, L.M. Keeping pace with a popular enzyme GGT. Diagnostic Medicine 1982; May/June: 1-8.
  •  Tietz, N.W. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia, Pa : WB Saunders Company, 1995 : 286.
  •  In-house test data. Accurex Biomedical Pvt. Ltd., 2002 
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