Glucose Hexokinase 200

Glucose Hexokinase 200

Product Details:

  • Shape other
  • Usage Autopure Glucose Hexokinase is a ready to use two liquid reagent system With Autopure Glucose Hexokinase the assay is linear upto 600 mgdL
  • Appearance Biochemistry Reagent
  • Taste Other
  • Smell Other
  • Physical Form Powder
  • Other Names Biochemistry Reagent
  • Supply Ability : 10000 Kit Per Day
3987 INR/Kit

Price And Quantity

  • 3987 INR/Kit
  • 1 Kit

Product Specifications

  • Autopure Glucose Hexokinase is a ready to use two liquid reagent system With Autopure Glucose Hexokinase the assay is linear upto 600 mgdL
  • Other
  • other
  • Biochemistry Reagent
  • Reagents
  • Other
  • Other
  • Powder
  • Biochemistry Reagent
  • Biochemistry Reagent
  • 18 Months
  • 100
  • ACC9064

Trade Information

  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description


  • Autopure Glucose Hexokinase is a reagent kit for quantitative determination of Glucose concentration in human serum or plasma based on Hexokinase method
  • Autopure Glucose Hexokinase is a ready to use two liquid reagent system
  • With Autopure Glucose Hexokinase the assay is linear upto 600 mgdL

Clinical Significance

Estimation of glucose concentration is important in the diagnosis and management of hyperglycaemia and hypoglycaemia Condition of Hyperglycaemia may occur as a result of diabetes mellitus patients intravenously receiving glucose containing fluids during severe stress and cerebrovascular accidents Hypoglycaemia may be the result of an insulinoma insulin administration inborn errors of carbohydrate metabolism or fasting

Reagent Storage Stability  Handling

Autopure Glucose  Hexokinase is a ready to use two liquid reagent system

Shelf life

Unopened reagents are stable till the expiry date indicated on the label when stored at 2   8 C and protected from light

OnBoard Reagent Stability

Once opened the reagents are stable up to 60 days at 2 8 C  if protected from light and contamination Open bottle stability may vary from analyzer to analyzer Do not freeze the reagent  

Components & Concentration of Working Solution

 Components                                                                                           Concentration

  • Hexokinase                                                                                       >1500 U/L 
  • G6PHD                                                                                              > 2000 U/L 
  • ATP                                                                                                       >1 mmol/L 
  • Coenzymes, stabilizers & excipients

Specimen Collection & Preservation

Collect sample using standard sampling tube. Serum or plasma (heparin or fluoride) is the specimen of choice. Serum or plasma should be separated from the cell within 30 minutes  as the rate of glycolysis is approximately 7mg/dL per hour at room temperature. Sodium fluoride is preferred as anticoagulant due to its antiglycolytic activity Glucose in samples is stable for 1 day if stored at 2 -8C


Fully automated system automatically calculates the Glucose concentration of each sample.


For calibration  it is recommended to use c.f.a.s. or Accucal or any other suitable calibrator material.  

Calibration frequency

The frequency of calibration is dependent on the analyzer. Re calibration is recommended

  •  Whenever the reagent lot is changed.
  • As per the requirement of quality control procedures.  

Quality Control

Each batch of Autopure Glucose (Hexokinase) is assayed with atleast six control sera   Precinorm, Precipath, Biorad I Biorad II, Accutrol normal and Accutrol Abnormal prior to release  .

To ensure adequate quality control  it is recommended that the laboratory should use a normal and an abnormal commercial reference control material. It should be realized that the use of quality control material checks both reagent and instrument functions together. 

If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:

  •  Proper instrument function  wavelength setting  light source and temperature control
  •  Cleanliness of probes and cuvettes.
  •  Bacterial contamination of wash water used by the instrument
  •  Expiry date of the reagent kit 

Expected Values


60 - 110 mg/dL (3.33 - 6.11 mmol/L)


< 145 mg/dL (< 8.05 mmol/L)

 Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own reference range. For diagnostic purposes, Glucose results should always be assessed in conjunction with the patients medical history clinical examination and other findings.

Performance Characteristics


With Autopure Glucose (Hexokinase) the assay is linear upto 600 mg/dL. Determine samples with higher concentration via the rerun function. On instruments without rerun function, manually dilute samples with higher concentration using normal saline (0.9% NaCl) or distilled/deionized water and repeat the assay. The result obtained should be then multiplied with dilution factor to obtain correct Glucose concentration


Reproducibility was determined using quality control sera as shown below: 

n = 20

Within run

Between run

Quality Control Material

Mean mg/dL

SD mg/dL


Mean mg/dL

SD mg/dL


Low Contro







High Contol







 Correlation Studies

A comparison of Glucose determination using Autopure Glucose Hexokinase and reference competitors product gave the following correlationmgdL

Linear Regression

  • y  10123x  14318
  • R2  09983


  • Tietz N W Ed Fundamentals of Clinical Chemistry W B Saunders Co Philadelphia 
  • CLSI Method Evaluation Protocols Clinical  Laboratory Standards Institute Wayne PA
  • Young DS Effects of Drugs on Clinical Laboratory Tests 3rd ed  AACC Press Washington 1990
  • Inhouse test data Accurex Biomedical Pvt Ltd 2018  
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