Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of Peroxidase (POD), oxidatively couples with 4-aminoantipyrine and phenol to produce red quinoneimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.). The intensity of the colour complex is directly proportional to the concentration of glucose in specimen.

REAGENT STORAGE & STABILITY & HANDLING

• The kit should be stored at 2-8 degree C and is stable till the expiry date indicated on the label.
• The reagent and standard are ready-to-use and are stable till expiry when stored at 2-8 C. DO NOT FREEZE THE REAGENT.
• The reagent should be stored only in the amber bottle provided to protect it from direct light. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air). Before use swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
• Over time, the reagent may develop a light pink colour, this is expected and does not affect the reagent performance.
• Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity discard the reagent.

 SPECIMEN COLLECTION & PRESERVATION

Blood should be collected in a clean dry container. Serum or plasma should be separated from the cells at the earliest possible (within 30 minutes), as the rate of glycolysis is approximately 7 mg% per hour at room temperature.

For plasma separation following anticoagulants may be used.

• EDTA 2 mg/ml of blood
• CITRATE 6 mg/ml of blood
• HEPARIN 200 IU/ml of blood
• OXALATE 3 mg/ml of blood
• SODIUM FLUORIDE 10 mg/ml of blood

• Fasting blood Glucose 60 to 110 mg%
• Postprandial Blood Glucose  145 mg%