HDL Cholestrol 100

HDL Cholestrol 100

Product Details:

  • Supply Ability : 10000 Kit Per Day
1018 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 1018 INR/Kit

Trade Information

  • EXW ABPL
  • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • certification : iso

Product Description

INTRODUCTION
  • Auto Zyme HDL-Cholesterol Precipitating Reagent is for use in conjunction with Auto Zyme Cholesterol Reagent - for enzymatic determination of HDL-Cholesterol in serum or plasma.
  • Compared to the conventional Ultra-centrifugation method, the precipitation method is simple and time saving; particularly when combined with single-step enzymatic Auto Zyme Cholesterol reagent.
PRINCIPLE

Phosphotungstate/Mg2+ precipitates chylomicrons, LDL and VLDL fractions. High Density Lipoprotein (HDL) fraction remains un effected in supernatant. Cholesterol content of HDL fraction is assayed using Auto Zyme Cholesterol.

PREPARATION OF WORKING SOLUTION

HDL - Cholesterol Precipitating Reagent is ready-to-use as supplied

COMPONENTS & CONCENTRATION OF PRECIPITATING REAGENT

Component

Concentration

Phosphotungstic acid

2.4 mmo1/1

Magnesium Chloride

40 mmo1/1

SPECIMEN COLLECTION  & PRESERVATION

Blood should be collected in a clean dry container. Fasting blood is preferred for HDL-Cholesterol assay.

Plasma should be separated immediately from the cells. For plasma separation any of the following anticoagulants may be used:

  • EDTA 10 mg/ml blood
  • HEPARIN 200 Um blood

HDL-Cholesterol value is stable in serum for 24 hours at 2-8 degree C and 30 days 

PROCEDURE

HDL separation

Prewarm at room temperature (25 - 30 degree C) the required amount of Precipitating Reagent and Auto Zyme Cholesterol working solution before use.

Perform the assay as given below. 

Pipette as follows : 

  • Serum/plasma: 0.5 ml
  • HDL-Precipitating reagent: 0.5 ml

Mix thoroughly and centrifuge at 4000 r.p.m. for 10 minutes in a common laboratory centrifuge (1800 x g) to obtain a clear supernatant.

HDL-Cholesterol determination

  • Reaction type: End-Point
  • Reaction time: 10 mins. at 37 degree C130 mins. at R.T. (25-30 degree C)
  • Wavelength: 510 nm. (505-530 nm.)
  • Zero setting with : Reagent Blank
  • Blank absorbance limit:  0.100 Abs.
  • Sample volume: 0.05 m1(50 gl)
  • Reagent volume : 1.0 ml
  • Standard concentration: 50 mg%
  • Linearity: 400 mg%
Manual assay procedure

Assay the supernatant for HDL-Cholesterol within 2 hours after centrifugation using working solution of Auto Zyme Cholesterol Reagent.

Incubation

Incubate the assay mixture for 10 minutes at 37 degree C or 30 minutes at room temperature (25-30 degree C). After completion of the incubation, measure the absorbance of assay mixture against blank at 510 nm. Final colour is stable for two hours, if not exposed to direct light.

EXPECTED VALUES

30 - 70 mg%

CLINICAL SIGNIFICANCE 

OF HDL - CHOLESTEROL

HDL - Cholesterol values below 30 mg% indicate towards the risk factor. The clinical risk factor is better represented by the ratio of Total Cholesterol to HDL Cholesterol in case of heart ailments.

Risk Factor

Total Chol. / HDL Chol

 

Female

Male

Average x 0.5

3.3

3.4

Average x 1.0

4.5

5.0

Average x 2.0

7.0

9.6

Average x 3.0

11.0

24.0

 Clinical interpretation

Criteria for the treatment of lipid disorders :

 

 

Treatment required

 

 

No

Depending on the
HDL and / or LDL
Cholesterol level

Yes

Total Cholesterol' (mg/100 ml )

< 200

200 - 300

 

Triglycerides (mg/100 ml )

< 200

> 200

 

 

 

Treatment required

 

 

No

 

Yes

HDL Cholesterol' (mg/100 ml )

> 35

 

< 35

LDL Cholesterol 2 (mg/100 ml )

< 150

 

> 190

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might effect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

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