Homocysteine 25

Homocysteine 25

Product Details:

  • Shape other
  • Appearance Biochemistry Reagent
  • Usage AutoPure Homocysteine is a reagent kit for quantitative determination of L homocysteine in human serum and plasma on automated clinical chemistry analyzers. AutoPure Homocysteine is a ready to use, two liquid reagent system.
  • Physical Form Liquid
  • Other Names Biochemistry Reagent
  • Smell Other
  • Taste Other
  • Supply Ability : 10000 Kit Per Day
43713 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 43713 INR/Kit

Product Specifications

  • Other
  • Other
  • 18 Months
  • Reagents
  • ACC90699
  • Liquid
  • Biochemistry Reagent
  • Biochemistry Reagent
  • Other
  • AutoPure Homocysteine is a reagent kit for quantitative determination of L homocysteine in human serum and plasma on automated clinical chemistry analyzers. AutoPure Homocysteine is a ready to use, two liquid reagent system.
  • other
  • 100
  • Biochemistry Reagent

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

Introduction
  • AutoPure Homocysteine is a reagent kit for quantitative determination of L homocysteine in human serum and plasma on automated clinical chemistry analyzers.
  • AutoPure Homocysteine is a ready to use, two liquid reagent system.
  • With AutoPure Homocysteine, the assay is linear up to 50 pmo1/1.
Principle
AutoPure Homocysteine assay is based on the measurement of co  substrate conversion product.

Oxidized Homocysteine from the sample is reduced to free Homocysteine which then reacts with SAM to form methionine and SAH. SAH is assessed by coupled enzyme reactions where in adenosine is formed. The adenosine formed is hydrolyzed into inosine and ammonia which reacts with glutamate dehydrogenase with concomitant conversions of NADH to NAD*. The concentration of Homocysteine is proportional to the amount of NADH converted to NAD+ and is measured as change in absorbance at 340 nm.

Reagent Storage, Stability & Handling

AutoPure Homocysteine is a ready-to-use, two liquid reagent system.

Shelf life

Stable till expiry date indicated on the label when stored at 2 - 8 C.

On  Board Reagent Stability

  • R1: 60 days at 2 - 8 C after opening.
  • R2 : 60 days at 2-8C after opening.

Protect the reagent from light and contamination.

Do not freeze the reagent.

 Specimen Collection & Preservation

  •  Collect sample using standard sampling tube. Fresh serum and EDTA/heparinised plasma are the specimens of choice.
  • Haemolysed, turbid or severely lipemic specimens are not recommended for this assay.
  • After separation of plasma from cells, Homocysteine is stable for at least 4 days at room temperature and for 2 weeks at 2 - 8 C.

Calculations

Fully automated system automatically calculates the homocysteine concentration of each sample

Application Sheet

Refer to application sheet for details. For additional system applications, contact our local Accurex representative.

 Calibration

For calibration, it is recommended to use AutoPure Homocysteine calibrator from Accurex Biomedical Pvt. Ltd. Other commercially available homocysteine calibrators have not been tested with this assay and cannot be supported by Accurex. Refer to the AutoPure Homocysteine calibrator kit package insert for a description of assignment procedures and instructions. Calibration materials have concentrations around medical decision level.

Calibration frequency

The calibration curve is stable for 11 days. Re - calibration is recommended

  • Whenever the reagent lot is changed.
  • As per the requirement of quality control procedures.

Quality Control

Each batch of AutoPure Homocysteine is assayed with multiple quality control sera prior to release.

To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.
If the control values fall outside the specified limits, each of the below criteria should be cross - checked and corrected:
  • Proper instrument function   wavelength setting, light source and temperature control.
  • Cleanliness of probes and cuvettes.
  • Bacterial contamination of wash water used by the instrument.
  • Expiry date of the reagent kit.

Expected Values

Serum/Plasma Homocysteine : < 15 pmo1/1

Note:

Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the homocysteine results should always be assessed in conjunction with the patient's medical history, clinical examinations and other findings.

 Performance Characteristics Linearity

With AutoPure Homocysteine, the assay is linear up to 50 pmo1/1. Determine samples with higher concentrations via the rerun function. On analyzers without rerun function, manually dilute the sample with higher concentrations using distilled /deionized water (e.g. 1 +4). Multiply the result by the appropriate dilution factor (e.g. 5).

Interference

There is no significant interference from samples containing up to 500 mg/dl of haemoglobin, 40 mg/dl of bilirubin, 1000 mg/dl of triglycerides, 10 mM ascorbic acid and 100 pM of cystathionine.

Precision

Reproducibility was determined using three levels of pooled human sera. The within run and between run reproducibility was determined as shown below :

Serum pool

Mean  umol/1

CV Within run

CV Between run

Low

7

4.5

5.87

Mid

15.6

3.04

5.51

High

29

2.4

2.57

 Co - Relation Studies

Acomparison of Homocysteine determination using AutoPure Homocysteine and 

Abbott AxSYM Immunochemistry System gave the following co-relation (pmo1/1):

Linear Regression

  • y = 1.003x+ 1.654
  • r = 0.985

References

  • Eikelboom J.W., etal, Ann. Intern. Med. 1999; 131:363-75.
  • Scott. J., Weir D.Q , J. Med. 1996; 89:561-3.
  • Nygard 0., N. EngL J. Med. 1997; 337(4): 230-6.
  • Seshadri S., etal., N. EngL J. Med. 2002; 346: 477-483.
  • McLean R. etal., N. EngL J. Med. 2004; 350: 2042-2049.
  • Refsum, H., Clinical Laboratory News. May2002 : 2-14.
  • Guttormsen, A.B., etal., J. Nutr. 1994 ;124(10) :1934-41.
  • Vilaseca etal., Clin. Chem.1997 ; 43 : 690-692.
  • Faure-Delanef etal., Am. J. Hum. Genet.1997 ; 60 : 999-1001.
Enter Buying Requirement Details
Email Id
Mobile number

Other Products in 'AutoPure Reagents' category



Back to top
ACCUREX BIOMEDICAL PVT. LTD. All Rights Reserved.(Terms of Use)
Developed and Managed by Infocom Network Private Limited.