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Lyophilized calibrator should be reconstituted by adding 1.0 ml of distilled or deionized water. Dissolve the contents of the vial by swirling gently to avoid the formation of foam. Do not shake. Let stand for 20 minutes at room temperature before use.
REAGENT STORAGE STABILITY & HANDLING
Perform the assay as given below:
Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.
SPECIMEN COLLECTION & PRESERVATION
Blood should be collected in a clean and dry container. Although serum is preferred, anticoagulants such as heparin, citrate, oxalate, EDTA and sodium fluoride do not have significant influence on the assay when they are used in their usual amounts. Specimen analysis should be done immediately after collection. Do not use the specimens that are repeatedly subjected to freezing and thawing.
If the HDL-C value exceeds the linearity limit of 185 mg/dl, then dilute the specimen suitably with normal saline and repeat the assay. In such case the assay value should be multiplied with the dilution factor to obtain the correct HDL-C value of the specimen.
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.