LDL Cholesterol Direct 40

LDL Cholesterol Direct 40

Product Details:

  • Shape other
  • Other Names Biochemistry reagent
  • Physical Form Liquid
  • Taste Other
  • Shelf Life 18 Months
  • Appearance Biochemistry reagent
  • Usage Biochemistry reagent
  • Supply Ability : 10000 Kit Per Day
9804 INR/Kit
X

Price And Quantity

  • 9804 INR/Kit
  • 1 Kit

Product Specifications

  • Other
  • other
  • ACC1452
  • Biochemistry reagent
  • Biochemistry reagent
  • Biochemistry reagent
  • 18 Months
  • Liquid
  • Other
  • Biochemistry reagent
  • Reagents
  • 100

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION

 

  •  Infinite LDL-C is a reagent set for direct quantitative determination  of LDL-Cholesterol in human serum or plasma based on Direct  Homogenous method. 
  • Direct measurement by lntinite LDL-C overcomes the limitation of  Friedewald formula thereby allowing accurate determination of LDL-C. 
  •  Infinite LDL-C is a ready-to-use, two liquid reagent system stable  till expiry. 
  • Infinite LDL-C is linear upto 450 mg/di. 
  • There is no significant interference in hyper-lipemic and hyper immunoglobulinemic samples.  

 

PRINCIPLE 

 

  • In the first step, HDL, VLOL & Chylomicrons are eliminated under specific  conditions.  In the second step, special reagents break up LDL and make LDL Cholesterol available for measurement.  

 

PREPARATION OF  WORKING SOLUTION

The reagents R1 and R2 are ready-to-use.

Lyophilized calibrator should be reconstituted by adding 1.0 ml of distilled or deionized water. Dissolve the contents of the vial by swirling gently to avoid the formation of foam. Do not shake. Let stand for 20 minutes at room temperature before use.

REAGENT STORAGE STABILITY & HANDLING

  • The reagent kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label.
  • Reconstituted calibrator is stable for 3 days at 2 degree  - 8 degree C. Reconstitution stability of the calibrator may be extended up to 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20 degree C
PROCEDURE
  • Reaction type : End Point (2 Step)
  • Reaction time : 5 + 5 mins.
  • Wavelength: 578 nm.
  • Flowcell temperature: 37 degree C
  • Zero setting with:  Distilled water
  • Sample volume: 0.01 ml (10 NI)
  • Reagent volume: 0.750 ml + 0.250 ml
  • Linearity : 185 mg/dl
Manual assay procedure

Perform the assay as given below:

 

Calibrator

Sample

R1

0.01 ml

0.01 ml

 

0.750 ml

0.750 ml

  • Mix and incubate for 5 minutes at 37 degree C. 
  • Measure the absorbance Al at 578 nm.

 

Calibrator

Sample

R2

0.250 ml

0.250 ml


EXPECTED VALUES

The expected value for serum HDL-Cholesterol are as follows:
  • Men : 30 - 70 mg/di
  • Women : 30 - 85 mg/di

Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.

SPECIMEN COLLECTION & PRESERVATION

Blood should be collected in a clean and dry container. Although serum is preferred, anticoagulants such as heparin, citrate, oxalate, EDTA and sodium fluoride do not have significant influence on the assay when they are used in their usual amounts. Specimen analysis should be done immediately after collection. Do not use the specimens that are repeatedly subjected to freezing and thawing.

PROCEDURE LIMITATIONS

If the HDL-C value exceeds the linearity limit of 185 mg/dl, then dilute the specimen suitably with normal saline and repeat the assay. In such case the assay value should be multiplied with the dilution factor to obtain the correct HDL-C value of the specimen.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

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