Lipase 25

Lipase 25

Product Details:

  • Shelf Life 18 Months
  • Physical Form Liquid
  • Shape other
  • Taste Other
  • Usage Biochemistry reagent
  • Other Names Biochemistry reagent
  • Classification Reagents
  • Supply Ability : 10000 Kit Per Day
5503 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 5503 INR/Kit

Product Specifications

  • other
  • Other
  • 100
  • Biochemistry reagent
  • Liquid
  • Reagents
  • 18 Months
  • Other
  • Biochemistry reagent
  • Biochemistry reagent

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION
  • Infinite Liquid Lipase is a reagent set for determination of lipase activity in human serum and plasma based on colorimetric method.
  • Infinite Liquid Lipase is a ready-to-use. two liquid reagent system stable till expiry.
  • Infinite Liquid Lipase is linear up to 300
  • Infinite Liquid Lipase can be determined in just 9 minutes at 37 degree C.
PRINCIPLE

The chromogenic lipase substrate 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester is cleaved by the catalytic action of lipase to form 1,2-o-dilauryt-rac-glycerol and an unstable intermediate glutaric acid-(6-methyl resorufin) ester. This decomposes spontaneously in alkaline medium to form glutaric acid and methyl resorufin. The lipase activity in the specimen is proportional to the rate of formation of methyl resorufin in the reaction and can be determined photometrically at 578 nm.

REAGENT STORAGE STABILITY & HANDLING

 

  • The reagents R1 & R2 are ready-to-use.
  • The reagent kit should be stored at 2 - 8 degree C and is stable till the expiry date indicated on the label.

 

Component

Concentration

BICIN buffer, pH 8.0

50 mmol/I

Colipase

mg/1

Sodium deoxycholate

1.6 mmo1/1

Calcium chloride

10 mmo1/1


SPECIMEN COLLECTION  & PRESERVATION

Blood should be collected in a clean dry container. Although serum is preferred, plasma with lithium heparin can be used. Samples with any visible haemolysis are not acceptable Lipase activity in serum/plasma is stable for 5 days at 2 - 8 degree C or for 24 hours at 20 - 25 degree C. Do not use specimens that are repeatedly subjected to freezing and thawing.

PROCEDURE

 

  • Reaction type : Kinetic
  • Reaction direction: Increasing
  • Wavelength:  578 nm.
  • Flowcelt temperature: 37 degree C
  • Zero setting with:  Reagent Blank
  • Delay time : 120 seconds
  • No. of readings: 3
  • Interval : 60 seconds
  • Sample volume:  0.01 ml (10 pl)
  • R1 volume: 0.8 ml (800 p1)
  • R2 volume: 0.2 ml (200 pl)
  • Linearity :  300 1U/I

 

EXPECTED VALUES

13 - 60 IU/1

Expected range varies from population to population. It is therefore  recommended that each laboratory should establish its own normal range.

PROCEDURE LIMITATIONS

If the lipase activity exceeds 3001U/I, dilute the specimen with normal saline and repeat the assay. The result obtained should then be multiplied with the dilution factor to obtain correct lipase activity.

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include instrument function, temperature control, cleanliness of glassware and accuracy of pipetting

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