Lipase 30

Lipase 30

8509 INR/Kit

Product Details:

  • Other Names Biochemistry Reagent
  • Physical Form Liquid
  • Taste Other
  • Usage AutoPure Lipase is a ready to use two liquid reagent system With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
  • Shape other
  • Shelf Life 18 Months
  • Smell Other
  • Supply Ability : 10000 Kit Per Day
  • Click to view more

Price And Quantity

  • 1 Kit
  • 8509 INR/Kit

Product Specifications

  • ACC9057
  • Biochemistry Reagent
  • Other
  • Liquid
  • Other
  • Biochemistry Reagent
  • AutoPure Lipase is a ready to use two liquid reagent system With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
  • other
  • 18 Months
  • Biochemistry Reagent
  • Other
  • Reagents
  • 100

Trade Information

  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description


  • AutoPure Lipase is reagent kit for direct quantitative determination of Lipase in human serum and plasma on automated clinical chemistry analyzers
  • AutoPure Lipase is a ready to use two liquid reagent system
  • With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI

The chromogenic Lipase substrate 12  o  dilauryl  rac  glycero  3 glutaric acid6methylresorufin ester is cleaved by the catalytic action of lipase to form 12  o  dilauryl  rac  glycerol and an unstable intermediate glutaric acid  6  methylresorufin ester This decomposes spontaneously in alkaline medium to form glutaric acid and methylresorufin
  • 12  o  dilauryl  rac  glycero  3  glutaric acid  6  methylresorufin easter
  • 1 2  o  dilauryl  rac  glycerol  Glutaric acid   6  methylresorufin ester
 The Lipase activity in specimen is directly proportional to the rate of formation of methylresorufin in the reaction and can be determined kinetically

 Reagent Storage Stability  Handling 

 AutoPure Lipase is a ready to use two liquid reagent 

Shelf life

Stable till the expiry date indicated on the label when stored at 28C 

On   Board Reagent Stability

  • R1 28 days at 2 8C after opening
  • R2  28 days at 2 8C after opening
  • Protect the reagent from light and contamination
  • Do not freeze the reagent 

Components & Concentration of Working Solution 

Components                                                                         Concentration

  • BICIN buffer, pH 8.0                                                                50 mmo1/1
  • Colipase                                                                                       > 1 mg/I
  • Sodium deoxycholate                                                                  1.6 mmo1/1                                                            
  • Calcium chloride                                                                        10 mmo1/1

 Components                                                                                          Concentration

  • Tartatate buffer, pH 4.0                                                                               50 mmo1/1
  • Taurodeoxycholate                                                                                     8.8 mmo1/1
  • DGGMR*                                                                                                  0.027 mmo1/1
  • Stabilizers, excipients & surface active agents.

 Specimen Collection  Preservation

Collect sample using standard sampling tube Serum heparinized plasma with Lithium or Sodium can be used Lipase activity in serumplasma is stable for 5 days at 28C and 24 hours at 2025C Centrifuge samples containing precipitate before performing the assay  

 Application Sheet 

 Refer to the application sheet for details For additional system applications contact our local Accurex representative


For calibration it is recommended to use Cfas or any other suitable calibrator material

 Calibration frequency

Re  calibration is recommended

  • Whenever the reagent lot is changed
  • As per the requirement of quality control   

 Quality Control

Each batch of AutoPure Lipase is assayed with atleast six quality control sera  Precinorm Precipath Biorad 1 Biorad 11 Accutrol Normal and Accutrol Abnormal prior to release

 To ensure adequate quality control it is recommended that the laboratory should use a normal and abnormal commercial reference control serum It should be realized that the use of quality control material checks both the reagent and instrument functions together

If the control values fall outside the specified limits each of the below criteria should be cross  checked and corrected

  • Proper instrument function  wavelength setting light source and temperature control
  • Cleanliness of probes and cuvettes
  • Bacterial contamination of wash water used by the instrument
  • Expiry date of the reagent kit

 Expected Values


Adults  13  60 IU1 02171002 pkatl at 37 C


Expected range varies from population to population It is therefore recommended that each laboratory should establish its own range For diagnostic purposes the Lipase results should always be assessed in conjunction with the patients medical history clinical examinations and other findings 

 Performance Characteristics


With AutoPure Lipase the assay is linear upto 300 IU1 501 pkatl Determine samples with higher concentrations via the rerun function On instruments without rerun function manually dilute samples with higher concentrations using 09 NaCI or distilleddeionized water eg 1  4 Multiply the result by the appropriate dilution factor eg 5 


 There is no significant interference in samples containing upto 60 mgdl of bilirubin and 500 mgdl of haemoglobin 


Reproducibility was determined using quality control sera as shown below

Within run

Between run

Quality Control Material

Means 1U/1

SD 1U/1


Means 1U/1

SD 1U/1


Low Control Serum







High Control Serum







 Co  Relation Studies

Acomparison of the Lipase determination using AutoPure Lipase and Infinite Lipase reagent gave the following corelation IU1

Linear Regression

  • y  0304x  656
  • r  0986
  • Syx 174

No of samples measured  72

The sample concentrations measured were between 5 and 661 IU1 


  • Tietz N W etal Lioase in Serum  the elusive enzyme An overview Clin Chem1993 39746756
  • Tietz NW etal Clinical guide to Laboratory Tests WB Saunders Philadelphia p 865 1995
  • Leybold A Jung W Imprtance of Colipase for the measurement of serum lipase activity Adv Clin EnzymoL 1986 46067
  • Pentaghini M etal Diagnostic value of four assays for lipase determination in serum A comparative reevaluation Clin Biochem 1991 24497503
  • Steinberg W M Goldstein SS Davies ND etal Diagnostic assays in acute pancreatitis Review Ann Intern Med 1985 102576580 
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