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Lipase 30
8509 INR/Kit
Product Details:
- Other Names Biochemistry Reagent
- Physical Form Liquid
- Taste Other
- Usage AutoPure Lipase is a ready to use two liquid reagent system With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
- Shape other
- Shelf Life 18 Months
- Smell Other
- Supply Ability : 10000 Kit Per Day
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Price And Quantity
- 1 Kit
- 8509 INR/Kit
Product Specifications
- ACC9057
- Biochemistry Reagent
- Other
- Liquid
- Other
- Biochemistry Reagent
- AutoPure Lipase is a ready to use two liquid reagent system With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
- other
- 18 Months
- Biochemistry Reagent
- Other
- Reagents
- 100
Trade Information
- EXW ABPL
- Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
- 10000 Kit Per Day
- 2 Days
- Yes
- packaging : Empity
- Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
- All India
- certification : iso
Product Description
Introduction
- AutoPure Lipase is reagent kit for direct quantitative determination of Lipase in human serum and plasma on automated clinical chemistry analyzers
- AutoPure Lipase is a ready to use two liquid reagent system
- With AutoPure Lipase the assay is linear upto 300 IU1 501 p katI
Principle
The chromogenic Lipase substrate 12 o dilauryl rac glycero 3 glutaric acid6methylresorufin ester is cleaved by the catalytic action of lipase to form 12 o dilauryl rac glycerol and an unstable intermediate glutaric acid 6 methylresorufin ester This decomposes spontaneously in alkaline medium to form glutaric acid and methylresorufin
- 12 o dilauryl rac glycero 3 glutaric acid 6 methylresorufin easter
- 1 2 o dilauryl rac glycerol Glutaric acid 6 methylresorufin ester
The Lipase activity in specimen is directly proportional to the rate of formation of methylresorufin in the reaction and can be determined kinetically
Reagent Storage Stability Handling
AutoPure Lipase is a ready to use two liquid reagent
Shelf life
Stable till the expiry date indicated on the label when stored at 28C
On Board Reagent Stability
- R1 28 days at 2 8C after opening
- R2 28 days at 2 8C after opening
- Protect the reagent from light and contamination
- Do not freeze the reagent
Components & Concentration of Working Solution
Components Concentration
- BICIN buffer, pH 8.0 50 mmo1/1
- Colipase > 1 mg/I
- Sodium deoxycholate 1.6 mmo1/1
- Calcium chloride 10 mmo1/1
R2
Components Concentration
- Tartatate buffer, pH 4.0 50 mmo1/1
- Taurodeoxycholate 8.8 mmo1/1
- DGGMR* 0.027 mmo1/1
- Stabilizers, excipients & surface active agents.
Specimen Collection Preservation
Collect sample using standard sampling tube Serum heparinized plasma with Lithium or Sodium can be used Lipase activity in serumplasma is stable for 5 days at 28C and 24 hours at 2025C Centrifuge samples containing precipitate before performing the assay
Application Sheet
Refer to the application sheet for details For additional system applications contact our local Accurex representative
Calibration
For calibration it is recommended to use Cfas or any other suitable calibrator material.
Calibration frequency
Re calibration is recommended
- Whenever the reagent lot is changed
- As per the requirement of quality control
Quality Control
Each batch of AutoPure Lipase is assayed with atleast six quality control sera Precinorm Precipath Biorad 1 Biorad 11 Accutrol Normal and Accutrol Abnormal prior to release
To ensure adequate quality control it is recommended that the laboratory should use a normal and abnormal commercial reference control serum It should be realized that the use of quality control material checks both the reagent and instrument functions together
If the control values fall outside the specified limits each of the below criteria should be cross checked and corrected
- Proper instrument function wavelength setting light source and temperature control
- Cleanliness of probes and cuvettes
- Bacterial contamination of wash water used by the instrument
- Expiry date of the reagent kit
Expected Values
SerumPlasma
Adults 13 60 IU1 02171002 pkatl at 37 C
Note
Expected range varies from population to population It is therefore recommended that each laboratory should establish its own range For diagnostic purposes the Lipase results should always be assessed in conjunction with the patients medical history clinical examinations and other findings
Performance Characteristics
Linearity
With AutoPure Lipase the assay is linear upto 300 IU1 501 pkatl Determine samples with higher concentrations via the rerun function On instruments without rerun function manually dilute samples with higher concentrations using 09 NaCI or distilleddeionized water eg 1 4 Multiply the result by the appropriate dilution factor eg 5
Interference
There is no significant interference in samples containing upto 60 mgdl of bilirubin and 500 mgdl of haemoglobin
Precision
Reproducibility was determined using quality control sera as shown below
Within run | Between run | |||||
Quality Control Material | Means 1U/1 | SD 1U/1 | CV | Means 1U/1 | SD 1U/1 | CV |
Low Control Serum | 13.3 | 1.52 | 1.1 | 140 | 1.85 | 1.4 |
High Control Serum | 456 | 2.53 | 0.6 | 475 | 2.32 | 0.9 |
Co Relation Studies
Acomparison of the Lipase determination using AutoPure Lipase and Infinite Lipase reagent gave the following corelation IU1
Linear Regression
- y 0304x 656
- r 0986
- Syx 174
No of samples measured 72
The sample concentrations measured were between 5 and 661 IU1
References
- Tietz N W etal Lioase in Serum the elusive enzyme An overview Clin Chem1993 39746756
- Tietz NW etal Clinical guide to Laboratory Tests WB Saunders Philadelphia p 865 1995
- Leybold A Jung W Imprtance of Colipase for the measurement of serum lipase activity Adv Clin EnzymoL 1986 46067
- Pentaghini M etal Diagnostic value of four assays for lipase determination in serum A comparative reevaluation Clin Biochem 1991 24497503
- Steinberg W M Goldstein SS Davies ND etal Diagnostic assays in acute pancreatitis Review Ann Intern Med 1985 102576580
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Contact Details
ACCUREX BIOMEDICAL PVT. LTD.
- 212, Udyog Mandir No. 17/C, Bhagoji Keer Marg, Mahim, Mumbai - 400016, Maharashtra, India
- Phone : 08045479256
- Fax : 91-22-66662223
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