Phosphorus 100

Phosphorus 100

Product Details:

  • Other Names Biochemistry reagent
  • Smell Other
  • Usage Auto Zyme Phosphorus is linear up to 20 mg%. Auto Zyme Phosphorus estimates phosphorus in just five minutes. Auto Zyme Phosphorus can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
  • Physical Form Liquid
  • Shelf Life 18 Years
  • Appearance Biochemistry reagent
  • Taste Other
  • Supply Ability : 10000 Kit Per Day
1015 INR/Kit
X

Price And Quantity

  • 1015 INR/Kit
  • 1 Kit

Product Specifications

  • Biochemistry reagent
  • Other
  • other
  • 18 Years
  • Reagents
  • Other
  • ACC74457
  • Biochemistry reagent
  • 100
  • Biochemistry reagent
  • Other
  • Liquid
  • Auto Zyme Phosphorus is linear up to 20 mg%. Auto Zyme Phosphorus estimates phosphorus in just five minutes. Auto Zyme Phosphorus can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.

Trade Information

  • EXW ABPL
  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

INTRODUCTION
  • Auto Zyme Phosphorus is a reagent set for determination of Inorganic phosphorus in serum based on UV - End Point method using Ammonium molybdate.
  • Auto Zyme Phosphorus is a single reagent system, ready-to-use.
  • Auto Zyme Phosphorus is linear up to 20 mg%.
  • Auto Zyme Phosphorus estimates phosphorus in just five minutes.
  • Auto Zyme Phosphorus can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
  • The shelf-life of Auto Zyme Phosphorus is 18 months.
PRINCIPLE

Inorganic Phosphorus reacts with Ammonium molybdate in strong acidic medium to form Phospho-lnorganic Molybdate complex. The absorbance of this complex is directly proportional to the phosphorus concentration.

PREPARATION OF  WORKING SOLUTION

The reagent is ready-to-use.

REAGENT STORAGE & STABILITY

  • The reagents are for in vitro diagnostic use.
  • Molybdate reagent & standard should be stored at temperature indicated on the bottle label.

COMPONENTS & CONCENTRATION  OF WORKING SOLUTION

Component

Concentration

Ammonium molybdate

0.3 mmol/l

Sulphuric Acid

1.0%

SPECIMEN COLLECTION  & PRESERVATION

  • Blood should be collected in a clean dry container. Neatly separated serum should be used. Plasma is not recommended as anticoagulants may cause false low results.
  • Phosphorus is stable for 7 days in neatly separated serum. If the estimation is not possible within 7 days then the specimen should be preserved at -10 degree C and should be used within 3 weeks.
PROCEDURE
  • Reaction type: UV - End Point
  • Reaction time:  5 mins. at 37 degree C
  • Wavelength : 340 nm.
  • Zero setting with: Reagent Blank
  • Blank absorbance limit:  0.300 Abs.
  • Sample volume: 0.01 ml (10 ul)
  • Reagent volume: 1.0 ml
  • Standard concentration:  5 mg%
  • Linearity: 20 mg/dl
Manual assay procedure

The reagents should be brought to room temperature prior to use. Perform the assay as given below:

1.0 ml procedure

 

Serum / Plasma

Standard

Blank

Molybdate Reagent

0.01 ml

0.01 ml

 

1.0 ml

1.0 ml

1.0 ml

Incubation

Incubate the assay mixture for 5 minutes at 37 degree C. After completion of incubation period measure the absorbance specimen and standard against blank at 340 nm. Final colour is stable for two hours if not exposed to direct light.

 EXPECTED VALUES

  • ADULTS :2.5 - 5.0 mg/dl
  • CHILDREN :4.0 - 7.0 mg/dl
PROCEDURE LIMITATIONS
  • Discard the working reagent if the absorbance of the same is more than 0.300 against distilled water at 340 nm.
  • If the phosphorus value exceeds linearity limit then dilute the specimen suitably with normal saline and repeat the assay. In such case the assay value should be multiplied by the dilution factor to obtain correct phosphorus value of the specimen.
  • Strong lipemic and haemolytic sera should not be used.
  • Contaminated glassware is the greatest source of error. Disposable plastic tubes and clean glasswares are recommended for the test.
  • The reagent contains sulphuric acid. Avoid contact with skin and mucous membrane. If you come in contact with the reagent wash thoroughly with water.
QUALITY CONTROL

To ensure adequate quality control, it is recommended that each assay should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might effect the performance of this test include proper instrument function, cleanliness of glassware and accuracy of pipetting.

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