Total Protein 100

Total Protein 100

Product Details:

  • Shelf Life 12 Months
  • Shape other
  • Usage In - vitro Diagnostics
  • Appearance Liquid
  • Physical Form Liquid
  • Other Names Biochemistry reagent
  • Taste Other
  • Supply Ability : 10000 Kit Per Day
536 INR/Kit
X

Price And Quantity

  • 1 Kit
  • 536 INR/Kit

Product Specifications

  • Liquid
  • Biochemistry reagent
  • Biochemistry reagent
  • ACC123
  • Other
  • 12 Months
  • Liquid
  • Reagents
  • In - vitro Diagnostics
  • other
  • Other
  • 100

Trade Information

  • EXW ABPL
  • Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • All India
  • certification : iso

Product Description

INTRODUCTION

  • Auto Zyme Total protein is a reagent set for determination of total protein based on Biuret method.
  • Auto Zyme Total protein is a single reagent system, ready-to-use.
  • Auto Zyme Total protein is linear up to 18 gm%.
  • Total protein can be determined in just 5 minutes.
  • Auto Zyme Total protein does not require serum blank for lipemic samples.
  • Auto Zyme Total protein can be used on any Colorimeter, Spectrophotometer, Discrete semiautomated and Automated analyzer. Programmed can be designed for any specific analyzer upon request.
  • The shelf-life of Auto Zyme Total protein is 18 months.

 

PRINCIPLE

Proteins react with cupric ions under alkaline pH to produce a colour complex. This colour complex absorbs light at 546 nm. (530-570 nm.). The intensity of the colour is directly proportional to the protein concentration in specimen.

PREPARATION OF  WORKING SOLUTION

The reagent is ready-to-use.

REAGENT STORAGE & STABILITY

  • The reagents are for in vitro diagnostic use.
  • Biuret reagent & standard should be stored at temperature indicated on the bottle label.

COMPONENTS & CONCENTRATION  OF WORKING SOLUTION

Component

Concentration

Cupric sulphate

7 mmo1/1

Potassium Iodide

6 mmo1/1

Tartarate

20 mmo1/1

Surfactant

0.05% w/v

Stabilizer

7 mmo1/1

SPECIMEN COLLECTION  & PRESERVATION

Blood should be collected in a clean dry container. Avoid the use of plastic or siliconized containers for blood collection which may prolong clotting time. Serum or plasma should be separated from the cells within 60 minutes.

PROCEDURE

  • Reaction type: End-Point
  • Reaction time: 5 mins. at 37 degree C
  • Wavelength:546 nm.(530-570 nm.)
  • Zero setting with : Reagent Blank
  • Sample volume:  0.01 ml (10 III)
  • Reagent volume: 1.0 ml
  • Standard concentration:  6 gm%
  • Linearity: 18 gm%

Manual assay procedure

Prewarm at room temperature (25-30 degree C) the required amount of working solution before use.

Perform the assay as given below :

1.0 ml procedure

 

Serum / Plasma

Standard

Blank

Working Solution

0.01 ml

0.01 ml

 

1.0 ml

1.0 ml

1.0 ml

 Incubation

Incubate the assay mixture for 5 minutes at 37 degree C. After completion  of incubation period measure the absorbance of specimen and  standard against blank. Final colour is stable for 8 hours if not exposed to direct light.

PROCEDURE LIMITATIONS

  • The working reagent is considered unsatisfactory and should not be used if it develops insoluble precipitate.
  • If the total protein content of a specimen exceeds 18 gm% dilute the specimen with an equal volume of saline and repeat the assay. Multiply the result by two to obtain correct value.
  • For Haemolyzed and Icteric samples (Bilirubin > 5 mg%) a saline blank should be run along with the assay. Read the absorbance of saline blank against distilled water and subtract it from sample absorbance.

 

QUALITY CONTROL

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

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