Total Protein 80

Total Protein 80

Product Details:

  • Shape other
  • Physical Form Liquid
  • Usage AutoPure Total Protein is ready to use, single liquid reagent system. With AutoPure Total Protein the assay is linear upto 18 g/dl (180 g/l)
  • Shelf Life 18 Months
  • Taste Other
  • Smell Other
  • Appearance Biochemistry Reagent
  • Supply Ability : 10000 Kit Per Day
670 INR/Kit

Price And Quantity

  • 670 INR/Kit
  • 1 Kit

Product Specifications

  • Biochemistry Reagent
  • Biochemistry Reagent
  • Biochemistry Reagent
  • 100
  • Other
  • Other
  • AutoPure Total Protein is ready to use, single liquid reagent system. With AutoPure Total Protein the assay is linear upto 18 g/dl (180 g/l)
  • Reagents
  • 18 Months
  • Other
  • ACC90677
  • other
  • Liquid

Trade Information

  • Paypal, Cash Against Delivery (CAD), Cash in Advance (CID), Cheque, Letter of Credit (L/C), Letter of Credit at Sight (Sight L/C)
  • 10000 Kit Per Day
  • 2 Days
  • Yes
  • packaging : Empity
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • All India
  • certification : iso

Product Description

  • AutoPure Total Protein is a reagent kit for direct quantitative determination of total protein in human serum and plasma on automated clinical chemistry analyzers.
  • AutoPure Total Protein is ready to use, single liquid reagent system.
  • With AutoPure Total Protein the assay is linear upto 18 g/dl (180 g/l).

  • Divalent copper ion reacts in alkaline solution with protein peptide bonds to form purple coloured biuret complex.
  • Protein + Cu2+ - Cu - protein complex. 
  • The intensity of purple colour formed is directly proportional to the concentration of the total protein in the specimen and is measured photometrically.

Reagent Storage, Stability & Handling

AutoPure Total Protein is ready to use, single liquid reagent system.

Shelf life

Stable till the expiry date indicated on the label, when stored at 15-25 C.

On  Board Reagent Stability.

  • 45 days at 2- 8C after opening. Protect the reagent from light and contamination.
  • Do not freeze the reagent.

Specimen Collection & Preservation
Collect sample using standard sampling tube. Serum, heparinized plasma or EDTA plasma can be used. Using plasma, results could be falsely elevated upto 0.4 g/dI due to fibrinogen. Avoid haemolysis since haemoglobin reacts as protein in the total protein assay.

Total protein in serum / plasma is stable for 3 days at 2- 8C and 6 months at  -20C. Centrifuge samples containing precipitate before performing assay.


  • Fully automated system automatically calculates the total protein concentration of each sample.
  • Results in g/I = Results in g/dI x 10

Application Sheet

Refer to the application sheet for details. For additional system applications, contact our local Accurex representative.


For calibration, it is recommended to use C.f. a.s or any other suitable calibrator material.

Calibration frequency

Re  calibration is recommended

  • Whenever the reagent lot is changed.
  • As per the requirement of quality control procedures 

 Quality Control

Each batch of AutoPure Total Protein is assayed with atleast six quality control sera   Precinorm, Precipath, Biorad I , Biorad 11, Accutrol Normal and Accutrol Abnormal prior to release

To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together.

If the control values fall outside the specified limits, each of the below criteria should be cross - checked and corrected:

  • Proper instrument function  wavelength setting, light source and temperature control.
  • Cleanliness of probes and cuvettes.
  • Bacterial contamination of wash water used by the instrument.
  • Expiry date of the reagent kit.
Expected Values
  • Serum/ Plasma
  • Adults : 6.3 - 8.4 g/dI (63  - 84 g/1)

Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the total protein results should always be assessed in conjunction with the patient's medical history, clinical examinations and other findings.

Performance Characteristics Linearity

With AutoPure Total Protein, the assay is linear up to 18 g/dI (180 g/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCI or distilled/deionized water (e.g. 1 + 3). Multiply the result by the appropriate dilution factor (e.g. 4).


There is no significant interference in samples containing upto 40 mg/di of bilirubin and 500 mg/di of haemoglobin. Haemoglobin interferes in the results as it reacts as a protein in total protein assay.


Reproducibility was determined using quality control sera as shown below:

Within run

Between run

Quality control material

Mean g/dl

SD g/dl


Mean g/dl


SD g/d


low control serum







High control serum







 Co - Relation Studies

A comparison of the Total Protein determination using AutoPure Total Protein reagent and AutoZyme Total Protein gave the following co relation (g/dI):

Linear Regression

  • y = -0.201 + 1.030x
  • r = 0.996
  • Sy.x = 0.10

No. of samples measured : 85

The sample concentrations measured were between 4 g/dI and 9 g/dl.


  • Tietz, N.W., ed. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia, Pa : W.B. Saunders, 1995: 518-522
  • Koller, A. Total Serum Protein. In Kaplan LA, Pesce AJ, eds. Clinical Chemistry Theory, Analysis and Correlation. St. Louis : Mosby Company, 1984 :1316 -1319.
  • Henri, R.J., et al "Clinical Chemistry - Principles and Techniques Harper & Row II ed (1974).
  • Flack, C.P. and Woolen, J.W. Clin Chem. 30,559 (1984).
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